运动预适应对C57BL/6小鼠溃疡性结肠炎的预防作用及其机制分析  

An analysis of the function of exercising preadaptation in preventing ulcerative colitis suffered by C57BL/6 mice and its mechanism

作　　者：代志军[1] 马延超[2] 

机构地区：[1]潍坊学院体育学院,山东潍坊261061 [2]洛阳师范学院体育学院,河南洛阳471022

出　　处：《体育学刊》2014年第3期140-144,共5页Journal of Physical Education

基　　金：河南省科技计划项目(122300410257)

摘　　要：为探讨运动预适应对溃疡性结肠炎(UC)的影响及其可能机制,将24只C57BL/6小鼠随机分为运动预适应组(Ex组)、模型组(DSS组)和安慰剂对照组(C组),每组8只。Ex组进行8周跑台运动,DSS组和C组安静状态饲养8周。随后Ex组和DSS组自由饮用质量分数为3.5%葡聚糖硫酸钠(DSS)溶液诱导小鼠UC模型,C组自由饮用同等量的蒸馏水,每天观察并记录疾病活动指数(DAI)。7 d后处死小鼠,取肠道炎症组织观察病理变化并进行炎症评分;实时荧光定量PCR(RT-qPCR)法检测白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)mRNA表达水平;Western blot法检测Toll样受体4(TLR4)、总NF-κB p65和核NF-κB p65蛋白表达水平。结果显示,DSS组出现典型的UC临床表现和组织病理学变化,DAI评分、组织炎症评分、IL-1β和TNF-αmRNA表达以及TLR4和核NF-κB p65蛋白表达水平均高于C组(P<0.01);Ex组症状和组织病理学变化较DSS组减轻,DAI评分、组织炎症评分、IL-1β和TNF-αmRNA表达以及TLR4和核NF-κB p65蛋白表达水平均低于DSS组(P<0.01);3组总NF-κB p65蛋白水平差异无显著性(P>0.05)。结果表明,长期中等强度运动预适应能够预防UC、减轻UC病情,其机制可能与抑制TLR4﹣NF-κB信号通路以及下调下游炎症因子基因表达有关。In order to probe into the effects of exercising preadaptation on ulcerative colitis and its possible mecha- nism, the authors divided 24 C57BL/6 mice randomly into an exercising adaptation group （Ex）, a model group （DSS） and a placebo control group （C）, each of which contained 8 mice, let the mice in group Ex exercise on a treadmill for 8 weeks, feed the mice in groups DSS and C in a calm condition for 8 weeks, then let the mice in groups EX and DSS drink a 3.5% dextran sulfate sodium （DSS） solution freely to induce a mouse UC model, let the mice in group C drink the same amount of distilled water freely, observed and recorded disease activity index everyday, and 7 days later, killed the mice, took out inflamed intestinal tissues, observed their pathological changes and gave them an inflammation score, measured interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） mRNA expression levels by means of real-time florescence quantification PCR （RT-qPCR）, as well as Toll-like receptor 4 （TLR4）, total nuclear factor-κβ （NF-κβ） p65 and nuclear NF-κβ p65 protein expression levels by means of Westernblot, and revealed the following findings： the mice in group DSS showed typical UC clinical manifestations and histopathological changes, their DAI score, tissue inflammation score, IL-1β and TNF-α mRNA expression levels as well as TLR4 and nuclear NF-κβ p65 protein expression levels were all higher than those of the mice in group C （P〈0.01）; the symptoms and histopathological changes of the mice in group Ex were alleviated as compared with the mice in group DSS, their DAI score, tissue inflammation score, IL-1β and TNF-α mRNA expression levels as well as TLR4 and nuclear NF-κβ p65 protein expression levels were all lower than those of the mice in group DSS （P〈0.01）; there was no significant difference in total NF-κβ p65 protein expression level between the mice in these three groups. The said findings indicate that long-term medium intensity exercising

关 键 词：运动医学 运动预适应 溃疡性结肠炎 信号转导 炎症因子 小鼠 

分 类 号：G804.5[文化科学—运动人体科学]