角质细胞生长因子联合粒细胞集落刺激因子对5.0Gy照射小鼠造血及免疫系统的保护作用  

Protective effect of G-CSF and KGF on hematopoietic and immune system mice irradiated with 5.0 Gy γ-ray

作　　者：于程程[1] 张鹏程[2] 邢志伟[1] 沈秀[3] 王小春[4] 赵欣然[1] 李进[1] 郝建秀[1] 吴红英[4] 曹永珍[2] 姜恩海[1] 

机构地区：[1]中国医学科学院 北京协和医学院放射医学研究所临床室,天津300192 [2]天津医科大学总医院放疗科 [3]中国医学科学院 北京协和医学院放射医学研究所药物室,天津300192 [4]中国医学科学院 北京协和医学院放射医学研究所生化室,天津300192

出　　处：《中华临床医师杂志（电子版）》2013年第7期97-100,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：卫生部行业基金(201002009);中国医学科学院放射医学研究所探索基金(ST1214)

摘　　要：目的本文研究角质细胞生长因子(KGF)联合粒细胞集落刺激因子(G-CSF)对急性放射病小鼠造血及免疫系统的保护作用。方法以6～8周龄C57BL/6小鼠为研究对象,经137Cs-γ射线一次性全身照射后,通过体重、脏器系数、外周血常规(WBC、RBC、Hb、PLT)、股骨有核细胞数(BMNC)、骨髓DNA含量、脾结节数(CFU-S)等指标检测其对受照小鼠血液、造血及免疫系统的影响。结果不同给药组对辐射小鼠造血及免疫系统均有一定的保护作用。联合组的体重恢复情况明显好于其他各组;与单纯IR组比较,联合组的胸腺系数差异有统计学意义(P<0.05)、脾系数有统计学差异(P<0.01);联合组的WBC(P<0.05)、RBC(P<0.05)、Hb(P<0.01)、PLT(P<0.01)差异有统计学意义;联合组的BMNC、骨髓DNA含量、CFU-S均有统计学差异(P<0.01)。结论本研究表明KGF联合G-CSF对辐射损伤小鼠有一定疗效。Objective To study the protection of hematopoietic and immune system on acute radiation sickness in mice with combined KGF ＋ G-CSF.Methods C57BL/6 mice of 6-8 weeks were selected to receive whole body irradiation of γ-ray,through body weights,organ indexes,white blood cells,red blood cells,blood platelets and concentration of hemoglobin in peripheral blood,bone marrow nucleated cells,bone marrow DNA contents,colony forming units of spleen,to detect its effect on blood,hematopoietic and immune system in irradiated mice.Results Administered by different groups for irradiated mice in hematopoietic and immune system had a protective effect.The weight restoration of combined group was significantly better than other groups; Compared with simple IR group,difference of combined group with thymus index had statistically significant （P ＜ 0.05）,spleen index had significant difference （P＜0.01） ;Combined group with WBC （P ＜0.05）,RBC （P ＜0.05）,Hb （P ＜0.01）,PLT （P ＜ 0.01） had statistically significant; Combined group with BMNC,bone marrow DNA and CFU-S had all significant difference （P ＜0.01）.Conclusions KGF in combination with C-CSF is of certain protective effect for radiation injury in C57BL/6 mice.

关 键 词：成纤维细胞生长因子 粒细胞集落刺激因子 造血系统 免疫系统 

分 类 号：R142[医药卫生—公共卫生与预防医学]