解酒药对大鼠心肌急性缺血再灌注损伤的保护作用及其机制的初步研究  

Cardioprotective role and its possible mechanism of the hangover medicine on myocardial ischemia-reperfusion injury in rats

作　　者：赵航天 陈学颖[2] 范凡[2] 刘湘玮[2] 王聪[2] 申程[2,3] 马鑫[2] 朱洪[2] 孙爱军[2] 葛均波[2] 

机构地区：[1]新乡医学院,河南省453003 [2]复旦大学附属中山医院 上海市心血管病研究所 [3]山东大学

出　　处：《中华临床医师杂志（电子版）》2013年第7期124-127,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(30971250);2012年教育部新世纪优秀人才支持计划

摘　　要：目的通过大鼠心肌缺血再灌注模型研究解酒药对缺血再灌注(IR)损伤的保护作用。方法雄性SD大鼠分为IR+生理盐水对照组、IR+解酒药组、IR+解酒药+cyanamide(乙醛脱氢酶2抑制剂,氨基氰,CH2N2)组。伊文思蓝和TTC染色评估心肌梗死面积,TUNEL法测定缺血再灌注区域心肌细胞凋亡情况,Western blot测定分析内质网应激相关蛋白Grp78和Chop含量变化以及凋亡相关蛋白Caspase12含量变化。结果解酒药减轻心肌梗死面积,而cyanamide的加入部分抵消了解酒药对心肌缺血再灌注的保护作用[IR+生理盐水对照组:(57.72±6.52)%;IR+解酒药组:(35.59±5.77)%;IR+解酒药+cyanamide组:(46.83±5.96)%;IR+生理盐水对照组vs.IR+解酒药组,P<0.01;IR+生理盐水对照组vs.IR+解酒药+cyanamide组,P<0.01;IR+解酒药组vs.IR+解酒药+cyanamide组,P<0.01]。Grp78、Chop、Caspase12蛋白水平于IR+生理盐水对照组、IR+解酒药组、IR+解酒药+cyanamide组中均无统计学差异(P>0.05)。结论在心肌缺血再灌注损伤早期,解酒药对心肌缺血再灌注损伤具有保护作用,且抑制ALDH2后此作用消失,但其机制可能不是通过调控ALDH2参与的内质网应激而实现的。Objective To investigate whether hangover medicine plays cardioprotective role in the rat model of myocardial ischemia-reperfusion injury,and to reveal the possible mechanism.Methods 200-250 g Sprague-Dawley rats were divided into 3 groups randomly,before myocardial ischemia and repedusion procedure,saline （n =6）,hangover medicine （n =6） or hangover medicine ＋ cyanamide were given respectively by oral gavage and intraperitoneal injection.Myocardial infarction ratio was calculated by Evans blue and triphenyltetrazolium chloride （TTC） staining,and myocardial apoptosis was evaluated by TUNEL assay.Expression of endoplasmic-reticulumstress-related proteins（Grp78,Chop）and apoptosis-related protein（Caspase12） were quantified by Western blot.Results The infarction size was significantly reduced by the treatment of hangover medicine [hangover medicine group vs.saline group,（35.59 ± 5.77） ％ vs.（57.72 ± 6.52） ％,P ＜ 0.01].However,when the hangover medicine was blocked,the protection was diminished [hangover medicine group vs.hangover medicine and cyanamide group,（35.59 ± 5.77） ％ vs.（46.83 ± 5.96） ％,P ＜ 0.01].Myocardial apoptosis and the levels of Grp78,Chop,Caspase12 were not significantly different between the three groups（P ＞ 0.05）.Conclusions In the early phase of myocardial ischemia and reperfnsion injury,hangover medicine provides myocardial protection,which can be blocked by ALDH2 inhibitor.This influence may not be implicated through ALDH2 initiated endoplasmic reticulum stress.

关 键 词：再灌注损伤 内质网 应激 细胞凋亡 解酒药 乙醛脱氢酶2 

分 类 号：R285.5[医药卫生—中药学]