Facile Fabrication of Aggregation-induced Emission Based Red Fluorescent Organic Nanoparticles for Cell Imaging  

作　　者：张锡奇 Xiao-yong Zhang Bin Yang 危岩 

机构地区：[1]Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology(Ministry of Education),Tsinghua University

出　　处：《Chinese Journal of Polymer Science》2014年第7期871-879,共9页高分子科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21134004,21201108 and 51363016);the National 973 Project(No.2011CB935700);China Postdoctoral Science Foundation(Nos.2012M520243,2013T60100)

摘　　要：A cyano-substituted diarylethlene derivative aggregation-induced emission （ALE） dye with two amino end-groups and 4,4＇-（hexafluoroisopropylidene）diphthalic anhydride were facilely incorporated into red fluorescent organic nanoparticles （FONs） via room temperature anhydride ring-opening polymerization under an air atmosphere. These obtained RO-HFDA FONs were characterized by a series of techniques including gel permeation chromatography, Fourier transform infrared spectroscopy, size distribution and zeta potential measurements, UV-Vis absorption spectrum, fluorescent spectroscopy and transmission electron microscopy. Biocompatibility evaluation and cell uptake behavior of RO-HFDA FONs were further investigated to explore their potential biomedical application. We demonstrated that such FONs showed high water dispersibility, stable uniform spherical morphology （150-200 nm）, broad excitation band （350-605 nm）, intense red fluorescence （627 nm） and excellent biocompatibility, making them promising for cell imaging applications.A cyano-substituted diarylethlene derivative aggregation-induced emission （ALE） dye with two amino end-groups and 4,4＇-（hexafluoroisopropylidene）diphthalic anhydride were facilely incorporated into red fluorescent organic nanoparticles （FONs） via room temperature anhydride ring-opening polymerization under an air atmosphere. These obtained RO-HFDA FONs were characterized by a series of techniques including gel permeation chromatography, Fourier transform infrared spectroscopy, size distribution and zeta potential measurements, UV-Vis absorption spectrum, fluorescent spectroscopy and transmission electron microscopy. Biocompatibility evaluation and cell uptake behavior of RO-HFDA FONs were further investigated to explore their potential biomedical application. We demonstrated that such FONs showed high water dispersibility, stable uniform spherical morphology （150-200 nm）, broad excitation band （350-605 nm）, intense red fluorescence （627 nm） and excellent biocompatibility, making them promising for cell imaging applications.

关 键 词：Cyano-substituted diarylethlene derivative Aggregation-induced emission Anhydride ring-opening polymerization Red fluorescent organic nanoparticles Cell imaging. 

分 类 号：TQ422[化学工程]