NOSTRIN在糖尿病性勃起功能障碍大鼠阴茎海绵体组织中的表达  

Expression of endothelial nitric oxide synthase traffic inducer in the cavernous tissues of diabetes mellitus erectile dysfunction rats

作　　者：权伟合[1,2] 王涛[2] 李明超[2] 饶可[2] 杨俊[2] 孟祥虎[2] 卢灿峰[2] 刘继红[2] 叶章群[2] 

机构地区：[1]湖北医药学院附属东风医院泌尿外科,湖北十堰442008 [2]华中科技大学同济医学院附属同济医院泌尿外科

出　　处：《中华临床医师杂志（电子版）》2013年第5期100-103,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的检测糖尿病性勃起功能障碍(ED)大鼠阴茎海绵体组织中内皮型一氧化氮合成酶运输转导物(NOSTRIN)的表达。方法将12周龄雄性SD大鼠20只随机分为2组,对照组8只,糖尿病组12只,链脲佐菌素诱导糖尿病性ED模型,8周后检测两组大鼠阴茎海绵体内压,并取阴茎海绵体组织行realtimeRT-PCR、Westernblot、免疫组化染色测定NOSTRIN、eNOS的表达。结果糖尿病组大鼠阴茎海绵体内压比造模前明显降低(P<0.01),而对照组无明显差异。与对照组比较,糖尿病组NOSTRIN表达升高,eNOS表达降低(P<0.01)。结论 NOSTRIN在糖尿病性ED大鼠阴茎海绵体中表达升高,eNOS表达降低。Objective To investigate the expression change of endothelial nitric oxide synthase traffic inducer（NOSTRIN） in the cavernous tissues of diabetes mellitus erectile dysfunction（DMED） rats.Methods Twenty male Sprague-Dawley rats were divided into Groups A （normal control,n =8）and B （DM group,n =12）,the diabetic models were made by intraperitoneal injection of strepozocin（STZ）.Eight weeks later,the erectile function of the rats was assessed through measuring the mean arterial pressure（MAP） and intracavemous pressure（ICP）.The expression changes of NOSTRIN and eNOS were detected in cavernous tissues by real time reverse transcription PCR,Western blot and immunohistochemical staining.Results ICP of group B was significantly lower than that of group A （P 〈0.01）.Compared with group A,the expression of NOSTRIN was markedly increased,the expression of eNOS significantly decreased（P 〈 0.01）.Conclusions The expression of NOSTRIN is increased and the expression of eNOS is decreased in the cavernous tissues of DMED rats.

关 键 词：勃起功能障碍 一氧化氮合酶 大鼠 

分 类 号：R587.2[医药卫生—内分泌]