大鼠颅脑损伤后脑NF-κB的活性和NF-κBmRNA表达的变化及意义  

The change and significance of the activity of NF-κB and the expression of NF-κB mRNA after traumatic brain injury in rats

作　　者：钱伟[1] 钱志远[1] 沈合春[1] 郑达理[1] 

机构地区：[1]苏州大学附属第二医院神经外科,江苏省215004

出　　处：《中华临床医师杂志（电子版）》2013年第5期117-119,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：苏州市科技发展计划指导项目(SYSD2011086)

摘　　要：目的观察颅脑损伤后不同阶段脑NF-κB的活性和NF-κBmRNA表达的变化,探讨其在颅脑损伤后继发性损伤中意义及相关机制。方法建立颅脑损伤模型,通过光镜观察颅脑损伤后病灶周围脑组织变化,采用免疫组化方法检测脑组织中NF-κB及TNF-α的表达情况,RT-PCR检测NF-κBmRNA表达情况。结果颅脑损伤后不同阶段NF-κB蛋白表达及mRNA表达明显高于对照组(P<0.05),并且NF-κB表达水平与TNF-α呈正相关关系(r=0.519,P<0.05),且与组织炎症损伤程度一致。结论颅脑损伤后各时间点脑NF-κB呈过度激活状态,与TNF-α水平的变化一致,且病理上颅脑损伤炎症损害严重程度相平行,这可能是颅脑损伤后继发性损伤发生的一个机制。Objective To observe the NF-κB activity and expression of NF-κB mRNA change after traumatic brain injury in rats,and to research the mechanism in secondary brain injury.Methods 48 male rats were randomly divided into Sham operation group（Sham group,n =6）and traumatic brain injury group（STBI group,n =42）.The rat TBI model was established by improved Feeney method.The pathological change was observed through HE staining,and the activity of NF-κB and TNF-α was judged by immunohistochemistry staining.The mRNA level of NF-κB was detected by RT-qPCR.Results The activity of NF-κB after traumatic brain injury was significantly higher（P 〈 0.05）,and the activity of TNF-α was increased （P 〈 0.05）.The activity of NF-κB and TNF-α were positively correlated（r =0.519,P 〈 0.05）.The expression of NF-κB in 3 h and 6 h were higher than that in the Sham group（P 〈0.05）,the rest had no significant difference（P 〉0.05）.Conclusions The NF-κB is excessively activated in traumatic brain injury,which is consistent with the change of TNF-α and the pathological change.The NF-κB may play an important role in secondary brain injury.

关 键 词：颅脑损伤 NF-ΚB 肿瘤坏死因子Α 继发性脑损伤 

分 类 号：R651.15[医药卫生—外科学]