Metallothioneins Induced by Antimony Accumulation in Earthworm Eisenia foetida Using Filter Paper Contact Method  

作　　者：Mengjiao Geng Shuxuan Liang Wei Liu Kai Wang Hanwen Sun 

机构地区：[1]College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China

出　　处：《Journal of Environmental Science and Engineering(A)》2013年第11期686-691,共6页环境科学与工程（A）

摘　　要：MTs （Eiseniafoetida metallothioneins） have been recently reported as biomarkers for environmental metal contamination, however no study regarding Sb （antimony） is available. This study aimed to explore possibility of MTs as a biomarker of Sb pollution. A contact method of filter paper was used to investigate Sb inducing MTs in Eiseniafoetida. Spectrophotometrical and SDS-PAGE （sodium dodecyl sulphate-polyacrylamide gel electropheresis） analyses were used to determine the nature of the induced MTs samples. Rabbit liver metallothioneins was used as a standard. The results showed that the molecular weight of induced protein was approximately 6-7 kDa. A characteristic absorption peak at 268 nm was observed, which was consistent with the standard. The amounts of Sb-MTs quantified by cadmium hemoglobin saturation method showed a significant positive relationship with increasing Sb exposure.The experiments prove that Sb can induce Sb-MTs in Eiseniafoetida as a biomarker of antimony pollution.

关 键 词：METALLOTHIONEINS Sb（III） Eiseniafoetida. 

分 类 号：Q513[生物学—生物化学] S859[农业科学—临床兽医学]