2型糖尿病大鼠骨骼肌葡萄糖转运蛋白-4和磷脂酰肌醇-3-激酶与内脂素的关系  

Effect of visfatin on GLUT4 mRNA and PI3K signalling pathway of skeletal muscle in diabetic rats

作　　者：魏元元[1] 张绍维[1] 王巍[1] 李鹏飞[2] 张玉敏[2] 罗雨葳 赵文州[1] 

机构地区：[1]解放军第202医院内分泌科,沈阳116044 [2]沈阳医学院环境卫生教研室

出　　处：《中华临床医师杂志（电子版）》2013年第12期106-108,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：辽宁省自然科学基金(20092085)

摘　　要：目的研究内脂素对糖尿病大鼠骨骼肌葡萄糖转运蛋白-4(GLUT4)和磷脂酰肌醇-3-激酶(PI3K)表达的影响并探讨其机制。方法健康雄性Wistar大鼠随机分为普通饲料组(N组)、高脂饲料组(H组)、普通饲料糖尿病模型组(N+S组)及高脂饲料糖尿病模型组(H+S组)。糖尿病模型组大鼠腹腔注射链脲佐菌素造模。造模成功8周后,两组糖尿病大鼠再分别随机分出一组为V+N+S组和V+H+S组,腹腔注射内脂素重组蛋白。采用实时定量RT-PCR技术检测骨骼肌GLUT4 mRNA表达,Western blot技术检测GLUT4和PI3K蛋白表达。结果 N+S组与N组、H+S组与H组大鼠骨骼肌GLUT4 mRNA,GLUT4和PI3K蛋白表达水平比较明显减低(P<0.05)。V+N+S组与N+S组、V+H+S组与H+S组比较均有所升高(P<0.05)。结论内脂素可能通过增加PI3K的蛋白表达影响GLUT4的基因表达,导致其蛋白表达增加,从而降低血糖。Objective To investigate the effect of visfatin on GLUT4 mRNA expression and PI3K protein expression in skeletal muscle of diabetic rats and explore its mechanism .Methods Wistar rats were randomly divided into four groups：normal diet feeding group （ N ） , high-fat diet feeding group （ H ） , normal diet feeding ＋streptozotocin（STZ）-induced diabetic group（N＋S）and high-fat diet feeding＋STZ-induced diabetic group（H＋S）. Diabetic rats were given intraperitoneal injection of small dose STZ to establish models of type 2 diabetes.Visfatin were intraperitoneally injected in partial rats（V＋N＋S,V＋H＋S）from group（N＋S）and group（H＋S）after 8 weeks of establishment of models .Western blotting were used to assess the protein expression of PI 3K and GLUT4. GLUT4 mRNA expression were measured with real time RT-PCR.Results GLUT4 mRNA levels,GLUT4 and PI3K protein expression were decreased（P〈0.05）in the Group（N＋S）compared with group N,and in the Group（H＋S） compared with group H,and increased（P〈0.05）in the Group（V＋N＋S）compared with group（N＋S）,and in the Group（V＋H＋S）compared with group（H＋S）respectively.Conclusion Our data show that visfatin may increase GLUT4 mRNA levels and protein expression and lower plasma glucose levels by increase PI 3K protein expression in skeletal muscle of diabetic rats .

关 键 词：糖尿病 2型 烟酰胺磷酸核糖基转移酶 葡萄糖转运体4型 1-磷脂酰肌醇3-激酶 

分 类 号：R587.1[医药卫生—内分泌]