角质细胞生长因子对小鼠放射性肠损伤的治疗作用  

Keratinocyte growth factor treatment prevents radiation-induced intestinal damage in a mouse model

作　　者：李佳[1] 王月英[2] 胡斌[3] 邢志伟[1] 于程程[1] 姜恩海[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所临床室,天津300192 [2]中国医学科学院北京协和医学院放射医学研究所生化室,天津300192 [3]承德市疾病预防控制中心预防医学门诊部

出　　处：《中华临床医师杂志（电子版）》2013年第13期130-132,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：卫生部行业基金(201002009);中国医学科学院放射医学研究所探索基金(1315)

摘　　要：目的本文研究角质细胞生长因子(KGF)对急性放射病小鼠肠损伤的治疗作用。方法将C57BL/6小鼠随机分为空白对照组、照射对照组、KGF低(1 mg/kg)、中(2 mg/kg)、高(4 mg/kg)剂量组5组,空白对照组给予假照射,其他4组给予全身一次性137CS-γ射线7.2 Gy照射,通过观察小鼠大体情况、检测小鼠肠组织蛋白浓度和NO含量及肠组织病理分析,探讨KGF对小鼠肠损伤的保护作用。结果 KGF给药组较照射对照组,体重恢复较快;KGF低、中、高剂量组较照射对照组肠组织蛋白浓度明显升高(P<0.05),KGF中、高剂量组较照射对照组NO浓度显著降低(P<0.05);肠组织病理情况较好。结论 KGF对小鼠放射性肠损伤有明显的治疗作用。Objective To study protective effect of keratinocyte growth factor（KGF） in radiation-induced intestinal injury in a mouse model. Methods The C57BL/6J mice were randomly divided into five groups：control group,radiation group and low, middle, high dose groups of KGF （ 1, 2,4 mg/kg）. The control group were sham- irradiated, other groups were given a single whole body irradiation at the dose of 7.2 Gy with 137CS-γ1 rays. Then body weight were measured, protein, NO concentrations and pathology were observed in intestinal. Results Compared with simple IR group, KGF treatment groups showed better status in body weight and pathology of intestinal, KGF low, middle dose groups with protein concentrations had statistically significant （ P 〈 0. 05 ）, KGF high dose group with protein concentrations had statistically significant（P 〈 0.01 ）, KGF middle,high dose groups with NO concentrations had statistically significant （ P 〈 0.05 ）. Conclusion KGF treatment could improve radiation-induced intestinal injury in a mouse model.

关 键 词：辐射 小鼠 角质细胞生长因子 

分 类 号：R818[医药卫生—放射医学]