骨髓间充质干细胞移植对大鼠脑出血后神经可塑性的影响  

Influence of neuroplasticity of rat cerebral hemorrhage by bone marrow mesenchymal stem cells transplantation

作　　者：单泓[1] 李建斌[1] 刘敏[1] 戚正[1] 王姣杰[1] 韩小改[1] 梁会涛 

机构地区：[1]河南省红十字血液中心成分室,郑州450000

出　　处：《中华临床医师杂志（电子版）》2013年第15期106-108,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：河南省医学科技攻关计划(201204120)

摘　　要：目的观察骨髓间充质干细胞(BMSCs)移植对大鼠脑出血后神经可塑性的影响。方法利用脑立体定位仪制作大鼠脑出血模型;将120只大鼠随机分成BMSCs移植组、模型组,每组各60只;利用透射电镜观察血肿周围神经组织超微结构及神经突触的变化并应用Simple-PCI图像分析系统,选定测量参数计算突触数量,突触界面曲率,突触后致密区宽度及突触间隙;利用免疫组化技术检测大鼠血肿周围神经组织中神经突触相关蛋白Shank1、Nestin的表达;利用Berderson评分标准对大鼠进行神经功能评价。结果透射电镜观察,BMSCs移植组脑出血灶周围可见大量新生神经元及神经胶质细胞,神经突触数量为16.27±2.14,神经突触界面曲率为1.57±0.04,突触后致密区为68.32±10.54,突触间隙为14.65±1.58,与模型组比较差异有统计学意义(P<0.01)。BMSCs移植组及模型组Shank1表达分别为:75.82±10.65、14.33±1.14,两者比较差异有统计学意义(P<0.01);BMSCs移植组及模型组Nestin阳性细胞表达分别为:68.87±7.46、12.64±0.07,两者比较差异有统计学意义(P<0.01)。BMSCs移植组神经功能评分明显降低,与模型组比较有统计学意义(P<0.05)。结论 BMSCs可促进大鼠脑出血后神经组织修复,增强神经可塑性,促进神经功能恢复。Objective To observe the influence of neuroplasticity of hemorrhage in rats ai^e~ transplantation of marrow mesenchymal stem cell. Methods The models were made by brain solid positioner. Rats were randomly divided into BMSCs group and control group.Observe the ultrastructure of nervous tissue and the change of neurosynaptic near hematoma by transmission electron microscope. Study the neurosynaptic quantificationally. The expression of Shankland Nestin proteins which were related with synapse were detected by using immunohistochemistry. Evaluate the neural function of rats. Results Much new neuron and neurogliocyte were observed around cerebral hemorrhage in BMSCs group by transmission electron microscope. The number of neurosynaptic increased obviously. The curvature of synaptic boundary magnified and dense area of postsynatic thicken. The gap of cynapse narrowed obviously. Compared with arntrol group, it was statistically significant （P〈0.01）. The expression of Shankl protein around cerebral hemorrhage increased in BMSCs group. Compared with control group, it was statistically significant. The expression of Nestin positive ceils around cerebral hemorrhage were detected in BMSCs group. There is no Nestin positive cells in control group, The neurological scores of BMSCs group was significantly decreased. Compared with control ~oup, it was statistically significant. Conclusion The BMSCs transplantation can repair the nervous tissue,strengthen the plasticity of nerve and promote the recovery of neural function.

关 键 词：脑出血 间质干细胞 神经元可塑性 

分 类 号：R651.1[医药卫生—外科学]