糖尿病大鼠口服载胰岛素纳米粒的降血糖实验  

Study On Hypoglycemic Effects of Nanoparticles Loaded With Insulin In Diabetes Rats After Oral Given

作　　者：白录军 周天 冯旭阳[2] 郑鹏飞[2] 徐瑞芬[3] 

机构地区：[1]兰州军区临潼疗养院第二疗养区,陕西临潼710600 [2]第四军医大学西京医院心脏内科,陕西西安710032 [3]陕西省人民医院麻醉科,陕西西安710068

出　　处：《现代生物医学进展》2014年第13期2430-2432,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(8100597;81070248)

摘　　要：目的:利用糖尿病大鼠模型,研究载有胰岛素纳米粒的降血糖作用。方法:雄性SD大鼠18只,禁食12 h后,尾静脉一次性注射5%四氧嘧啶40 mg/kg制备大鼠糖尿病模型。超声条件下,制备含胰岛素纳米粒,纳米悬液的胰岛素浓度为2 U/ml,于4℃冷藏保存。大鼠随机分为三组,口服胰岛素组(A组)、载胰岛素纳米粒组(B组)、皮下注射胰岛素组(C组)。分别于灌胃前、灌胃后0.5,1,2,4,8,12 h取血测定血糖。结果:口服载胰岛素纳米粒后能显著降低大鼠的血糖水平,起效时间晚于皮下注射胰岛素,但作用时间长久;而口服等量普通胰岛素血糖无明显变化。表明HTCC-ALG/OREC纳米粒在体内对胰岛素具有保护作用。结论:载胰岛素纳米粒具有一定的降血糖作用和缓释效果,是具有很好应用前景的口服蛋白质给药载体。Objective： To investigate hypoglycemic effects of nanoparticles loaded with insulin in diabetes rats after oral given. Methods： 18 male SD rats were fasted for 12 hours, and diabetic rat model were prepared by tail vein injection with alloxan of 5% 40 mg/kg. Nanoparticles loaded with insulin were prepared by ultrasonic. Nanosuspension with the concentration of 2 U/ml, were conserved in the fefrigerator at 4℃. Then randomly divided into three groups： oral given insulin（group A）, oral given nanoparticles loaded with insulin group（group B） and subcutaneous injection insulin（group C）. Glucose level were monitored respectively before and by gastric lavage after 0.5, 1, 2, 4, 8, 12 h. Results： The blood glucose level in rats significantly reduced after oral given for the nanoparticles loaded with insulin, and the onset time was later than the subcutaneous insulin injections, but the effect time was longer. No obvious change was found in blood glucose after oral given for the insulin. It is proved that nanoparticles played a protective role in the body. Conclusion： The nanoparticles loaded with insulin can reduced the glucose level, and the sustained release effect was realized, they have been used as drug carrier for protein.

关 键 词：胰岛素 纳米粒 口服 

分 类 号：Q95-3[生物学—动物学] R587.1[医药卫生—内分泌]