开唇兰属植物的种间蛋白质组差异  

Proteomic Difference of Anoectochilus Interspecies

作　　者：李雅潇[1] 高川[2] 张军[3] 

机构地区：[1]武警后勤学院药物化学教研室天津市职业与环境危害防制重点实验室,天津300162 [2]北京药物化学研究所,北京102205 [3]解放军306医院,北京100101

出　　处：《现代生物医学进展》2014年第14期2661-2666,2660,共7页Progress in Modern Biomedicine

基　　金：武警后勤学院青年基金(WYQ201106)

摘　　要：目的:通过差异蛋白峰的比较和指纹图谱的对照,分析开唇兰属五种金线莲的种间蛋白质组差异,初步探索了这种差异在品种鉴定中的应用并推断出品种之间的亲疏关系。方法:采用SELDI-TOF-MS方法研究了开唇兰属五种金线莲的种间蛋白质组差异。结果:通过SELDI-TOF-MS方法对开唇兰属五种金线莲种间蛋白质组谱图进行的分析结果表明,属内种间存在蛋白质组差异和特征蛋白质峰,据此建立了属内五种植物的蛋白质组指纹图谱,并依据蛋白质组的差异推测了属内五种金线莲的亲疏关系。结论:依据SELDI-TOF-MS鉴定结果,找到了开唇兰属五个物种间的蛋白差异,并基于差异蛋白数据分析了品种遗传变异性,结果表明地域和气候会对品种的变异产生一定的影响。Objective： To study the proteomic difference of Anoectochilus interspecies by the mean of comparing the differences of proteomic finger printings, and to explore preliminary the application of the proteomic differences in species identification. Methods： The proteomic difference of Anoectochilus interspecies are studied by means of surface enhanced laser desorption ionization time of flight mass spectrography. Result： Results showed that there are proteomic differences and specific proteins peaks among Anoectochilus interspecies. Moreover, proteomic finger printings of five Anoectochilus species are established for identifying species, and genetic relationships of five species within Anoectochilus have been deduced according to the proteomic differences among five species. Conclusion： According to the result of SELDI-TOF-MS, the proteomic differences of Anoectochilus interspecis were discovered. The analysis based on the proteomic differences shows the effect on the interspecies variation by location and climate.

关 键 词：金线莲 SELDI 蛋白质组 指纹图谱 

分 类 号：Q945.79[生物学—植物学]