拟南芥AtLCR23基因组织表达分析  

Expression Analysis of AtLCR23 Gene in Arabidopsis thaliana

作　　者：易力雄[1] 郭磊[2] 周雅智 刘春林[2] 阮颖[1] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]湖南农业大学农学院,长沙410128

出　　处：《作物研究》2014年第3期246-250,共5页Crop Research

基　　金：国家自然科学基金项目(31071455)

摘　　要：AtLCR23是拟南芥LCR基因家族中一个功能未知基因。目前有限的研究表明,拟南芥LCR家族中部分蛋白参与了植物防御机制。本研究通过半定量RT-PCR检测,AtLCR23主要在开放的花和幼嫩果荚中表达。构建pAtLCR23::AtLCR23:GUS融合表达载体,并对转基因植株进行组织化学染色,结果表明:AtLCR23基因特异性表达于已授粉的花和幼嫩果荚的柱头,结合生物信息学分析,推测AtLCR23的功能可能与花粉识别相关。AtLCR23 is a function unknown gene which belong to LCR families in Arabidopsis. Currently limited researches showed a part of LCR proteins were involved in plant defense mechanisms. Semi-quantitative RT-PCR confirmed AtLCR 23 mainly expressed in opening flowers and young siliques. Construction of pAtLCR23：：AtLCR23：GUS reporter line and histochemical staining of transgenic plants showed that AtLCR23 was specific expressed in stigma of pollinated flowers and young siliques. Combined with bioinformatics analysis,the results showed the function of AtLCR23 may be associated with pollen identification.

关 键 词：拟南芥 AtLCR23 基因表达 RT—PCR GUS染色 

分 类 号：Q786[生物学—分子生物学]