格列美脲含量测定方法的改进  

Improvement of the assay method of glimepiride

作　　者：郭明兴[1] 

机构地区：[1]山东省卫生教育培训中心,山东济南250014

出　　处：《药学研究》2013年第9期507-508,共2页Journal of Pharmaceutical Research

摘　　要：目的 改进格列美脲原料含量测定的方法.方法 采用高效液相色谱法,Agilent Zorbax SB-C18色谱柱（4.6 mm×250 mm,5 μm）,以磷酸二氢钠溶液（取0.5 g磷酸二氢钠,加水500 mL溶解,用磷酸调pH至2.5）-乙腈（500∶ 500）为流动相,流速1.0 mL·min-1,检测波长为228 nm.结果 系统适用性符合要求,格列美脲的定量限为0.1 ng,在87.92～1 318.80 ng范围内线性关系良好（r=1.000 0）.平均回收率为100.3%,RSD为0.8%（n=9）.结论 建立的方法准确、灵敏,可代替滴定法用于格列美脲的含量测定.Objective To improve the determine method for glimepiride. Methods The determination was performed on an Agilent Zorbax SB - Cls column（4. 6 mm × 250 mm,5μm） , and the mobile phase consisted of 500 mL sodium dihydrogen phosphate solution （ dissolve 0. 5 g of sodium dihydrogen phosphate in 500 mL of water and adjust to pH 2. 5 with phosphoric acid） and 500 mL of acetonitrileat the flow rate 1.0 mL· min-1. The detection wavelenth was 228nm. Results The system suitability met the requirements. The LOQs of glimepiride was 0. 1 ng. The linearity was found in the rang of 87. 92 - 1 318. 80 ng（ r = 1. 000 0 ）. The corresponding average recovery was 100. 3% with RSD 0. 8% （n = 9 ）. Conclusion The method was sensitive and accurate, and suitable for the assay of glimepirede.

关 键 词：格列美脲 含量测定 滴定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]