冠心病心绞痛寒凝血瘀证大鼠心肌组织形态学变化  

The histomorphology changes of myocardium in rats of angina pectoris with the syndrome of congealing cold with blood stasis

作　　者：王朋[1] 杨明会[2] 李绍旦[2] 霍旺[2] 刘毅[2] 张俊修[2] 

机构地区：[1]北京中医药大学第一临床医学院,北京100700 [2]解放军总医院中医研究所,北京100853

出　　处：《吉林中医药》2014年第4期396-398,共3页Jilin Journal of Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2012CB518601)

摘　　要：目的观察冠心病心绞痛寒凝血瘀证大鼠心肌组织形态学改变。方法采用垂体后叶素大剂量静脉注射建立冠心病心绞痛寒凝血瘀证动物模型,监测心电图变化;通过心肌组织HE染色、Nagar-Olsen特殊染色及电镜技术手段,观察心肌组织病理形态学的改变。结果与正常组相比,模型组大鼠光镜下心肌组织HE染色切片未见明显的形态学改变;Nagar-Olsen特殊染色可见大片红染的阳性反应区;透射电镜下可见肌纤维排列紊乱,线粒体聚集、肿胀,部分膜破裂,嵴降解等超微结构变化。结论冠心病心绞痛寒凝血瘀证大鼠心肌组织出现缺血病理形态学改变。Objective To observe the histomorphology changes of myocardium in rats of angina pectoris with the syndrome of congealing cold with blood stasis. Methods The animal model of angina pectoris with syndrome of congealing cold with blood stasis was established in rats by single high-dose intravenous injection of pituitrin. Electrocardiogram were monitored and the histomorphology changes of myocardium were observed by HE staining,Nagar-Olsen staining and transmission electron microscopy. Results Compared with the normal group,myocardial tissue in the model group displayed no significant morphological changes by HE staining while large areas of myocardium were dyed red by Nagar-Olsen staining under microscopy. Karyopyknosis and margination of the chromatin in some myocardial cells were observed under transmission electron microscopy. The mitochondria of some myocardial cells were congregated and swollen with some ruptured mitochondrial membrane and disrupted mitochondrial ridge. Conclusion The myocardium in rats of angina pectoris with syndrome of congealing cold with blood stasis displayed pathomorphism changes of ischemiainjury.

关 键 词：冠心病心绞痛 寒凝血瘀证 组织形态学 超微结构 大鼠 

分 类 号：R256.2[医药卫生—中医内科学]