趋化因子受体CXCR7在人早孕期滋养细胞和蜕膜基质细胞的表达  

Expression of chemokine receptor CXCR7 in human first-trimester trophoblasts and decidual stromal cells

作　　者：周雯慧 任亮[1] 刘越[3] 张元珍[1] 

机构地区：[1]武汉大学中南医院生殖医学中心,430071 [2]北京朝阳医院生殖医学中心 [3]武汉大学中南医院肿瘤科,430071

出　　处：《中华临床医师杂志（电子版）》2013年第4期49-51,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金项目(81270753;30801240);湖北省自然科学基金创新群体项目(2008CDA008)

摘　　要：目的研究趋化因子受体CXCR7在人早孕期母-胎界面的表达特征。方法收集人早孕期绒毛与蜕膜组织,免疫组织化学技术分析趋化因子受体CXCR7及CXCR4在母-胎界面的表达情况;分别分离培养人早孕期滋养细胞及蜕膜基质细胞,免疫细胞化学技术分析CXCR7和CXCR4在两种细胞的表达。结果人早孕期绒毛与蜕膜组织及体外分离培养的人早孕期滋养细胞、蜕膜基质细胞均可观察到特异性CXCR7和CXCR4阳性染色,但两种细胞CXCR7染色强度(平均光密度值)均略低于CXCR4,差异有统计学意义(P<0.05)。结论人早孕期滋养细胞及蜕膜基质细胞共表达趋化因子受体CXCR7和CXCR4,提示CXCR7可能在母-胎免疫微环境形成中也起重要作用。Objective To explore the expression of chemokine receptor CXCR7 at the materno-fetal interface during human first-trimester pregnancy. Methods Human first-trimester pregnancy villous and decidual tissues were collected, and primary trophoblast cells （TC）and dedidual stromal cells （DSC）were isolated and cultured. The protein expression of CXCR7 and CXCR4 were examined by immunohistochemistry （ IHC ） and immunocytochemistry（ICC） ,respectively. Results IHC and ICC disclosed specific staining for both CXCR7 and CXCR4 protein in TC and DSC ,respectively. However,the mean density of CXCR7 in TC and DSC was significantly lower than that of CXCR4 （ P 〈 0.05 ）. Conclusions CXCR7 and CXCR4 are co-expressed in human first-trimester pregnancy TC and DSC,which contributes to the establishment of materno-fetal immune microenvironment.

关 键 词：母-胎界面 滋养细胞 蜕膜基质细胞 CXCR7 CXCR4 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]