大豆空间诱变突变体的RAPD分析  

RAPD Analysis of Soybean Mutants by Space Mutation

作　　者：赵曦[1] 王广金[1] 李铁[1] 刘丽艳[1] 谭巍巍[1] 陆杰[1] 付亚书 刘昭军[1] 

机构地区：[1]黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨150086 [2]黑龙江省农科院绥化分院,黑龙江绥化152052

出　　处：《核农学报》2014年第5期772-776,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家转基因重大专项基金(2008ZX08009-003)

摘　　要：本研究应用RAPD技术在分子水平进行大豆空间诱变突变体的差异性分析,为大豆空间诱变育种提供理论基础。首次应用RAPD标记对大豆空间诱变的12个品系及4个原始对照种进行多态性分析。随机选用的70个RAPD引物对原始对照种及12个空间诱变品系进行扩增,共获得247条DNA谱带,其中多态性谱带39条,总多态性位点比率为15.8%,品系间多态性位比率在1.43%-10%之间,扩增片段长度在250-2100bp之间。因此,通过空间诱变能够在DNA水平上导致大豆遗传物质变异,并且RAPD是一种研究植物空间诱变材料的较有效的分子生物学方法。This report is to research the differences of space-induced soybean mutant lines in DNA level by random amplified polymorphic amplified fragments（ RAPD） technology for the sake of providing theoretical basis for crop spaceflight mutation breeding. Polymorphism analysis was performed between twelve mutant lines and their original control varieties by random amplified polymorphic amplified fragments（ RAPD） technology for the first time. Twelve mutantlines and their original control varieties were analyzed by randomly selected 70 of RAPD primers. Thirty-nine were polymorphic in 247 DNA bands,which were generated from twelve samples. The total degree of gene polymorphism was 15. 8%,and polymorphism rate among samples was between 1. 43% and 10%,and fragment amplified was between 250- 2100 bp. Therefore,space mutagenesis could result in DNA variation of soybean,and RAPD is also an efficient molecular biological method for space mutagenesis analysis.

关 键 词：大豆 空间诱变 RAPD 多态性 

分 类 号：S565.1[农业科学—作物学]