抗牛多杀性巴氏杆菌高免卵黄抗体IgY的制备及间接血凝检测方法的建立  

Preparation and Establishment of the Indirect Haemagglutination of Highly Yolk Antibody IgY Against Bovine Pasteurella Multocida

作　　者：高家登[1] 剡根强[1] 王静梅[1] 杨铭伟[1] 王超丽[1] 杨龙龙[1] 

机构地区：[1]石河子大学动物科技学院,石河子832000

出　　处：《中国奶牛》2014年第9期26-29,共4页China Dairy Cattle

基　　金：国家科技计划支撑课题(2012BAD43B02);奶牛规模化养殖主要疾病综合防控技术集成与示范

摘　　要：本研究通过自制牛源荚膜血清A型多杀性巴氏杆菌灭活菌苗免疫产蛋鸡,采用卡拉胶结合硫酸铵沉淀法提取卵黄抗体IgY,并采用间接血凝方法检测抗体效价。结果表明,抗原致敏红细胞的最佳浓度是800μg/mL,免疫后第7周抗体效价达到高峰,效价为1:1024,高效价持续5周开始下降,测定提取的IgY浓度为8.258mg/mL,无菌检测及动物安全性实验表明制备的卵黄抗体安全可靠。本研究制备了抗牛多杀性巴氏杆菌卵黄抗体,为防治由荚膜血清A型多杀性巴氏杆菌所致的犊牛肺炎提供了新的手段。By the preparation and establishment of the indirect hemagglutination of highly yolk antibody against bovine capsular serotype A pasteurella multocida（Pm）, the hens were immunized with the inactivated vaccine of bovine Pm. The antibody titer was tested by indirect hemagglutination, IgY were extracted by carrageenan and salt precipitation with ammonium sulfate. The result showed that the best antigen concentration was 800μg/mL, IgY antibody titer reached the peak in the 7th week after the first immunized and titer of 1：1024 and last for 5 weeks. The IgY concentration obtained from egg yolk was 8.258mg/mL. Sterility detection and animals safe experiment results showed that the egg yolk antibodies were safe. Against bovine pasteurella multocida IgY was prepared, it also provided a new immune preparations for the prevention and control of the capsular serotype A pasteurella multocida pneumonia in calves.

关 键 词：牛多杀性巴氏杆菌 卵黄抗体(IgY) 间接血凝试验 

分 类 号：S858.23[农业科学—临床兽医学]