二甲基-β-丙酸噻亭对哲罗鱼生长性能和蛋白质代谢的影响  

Effects of dietary dimethyl-β-propiothetin supplementation on growth performance and protein metabolism of Hucho taimen

作　　者：王常安[1,2] 徐奇友[1] 李晋南[1] 赵志刚[1] 罗亮[1] 王洋[1] 尹家胜[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]东北林业大学野生动物资源学院,黑龙江哈尔滨150040

出　　处：《中国水产科学》2014年第3期541-548,共8页Journal of Fishery Sciences of China

基　　金：农业部公益性行业科研专项经费项目(201003055);国家科技支撑计划项目(2012BAD25B10);中央级公益性科研院所基本科研业务费项目(201103)

摘　　要：本研究旨在探讨二甲基-β-丙酸噻亭（DMPT）对哲罗鱼（Hucho taimen）生长性能、肌肉成分和蛋白质代谢的影响。选取1800尾初始体质量为（4．20±0．02）g的健康幼鱼，随机分为6组，设1个对照组，5个处理组，每组3个重复，每重复100尾。各处理组分别在基础饲料中添加0．02％、0．05％、0．1％、0．2％和0．5％的DMPT，对照组投喂基础饲料。室内流水饲养周期为56d。结果表明，饲料中DMPT添加量为0．2％时，哲罗鱼增重率和摄食率达最大值；以DMPT添加量和增重率进行二次曲线回归分析，得出DMPT最适添加量为0．24％。DMPT添加量为0．05％时，血清总蛋白、白蛋白和球蛋白水平显著提高（P〈0．05）；添加0．2％DMPT时，血清总蛋白、球蛋白水平和谷草转氨酶水平显著提高（P〈0．05）；添加水平0．02％～0．5％时，哲罗鱼肝和肌肉蛋白质水平显著升高（P〈0．05），胃、肠道蛋白酶活性显著升高（P〈0．05）；添加水平0．05％时，脏体指数显著降低（P〈0．05）；各处理组肌肉脂肪和灰分含量均与对照组差异不显著（P〉0．05），各组间血清谷丙转氨酶、肌肉氨基酸组成及含量未呈显著差异（P〉0．05）。综上可见，饲料中添加0．2％DMPT可明显提高哲罗鱼生长性能，添加0．02％～0．5％DMPT可增加哲罗鱼肌肉和肝蛋白储存。We evaluated the effects of dimethyl-β-propiothetin （DMPT） on growth, proximate muscle composition, and protein metabolism in Hucho taimen. Juvenile fish （n=1 800, average initial body weight： 4.20±0.02 g） were cultured in a flow through system. The fish were randomly divided into six triplicate groups of 100 fish and fed diets containing different levels of DMPT （0, 0.25%, 0.5%, 0.75%, and 1.0%） for 56 d. Weight gain rate was highest at 0.2% DMPT supplementation （P〈0.05）. The optimum supplementation was 0.24% based on a polynomial regression analysis between dietary DMPT level and weight gain rate. Serum total protein, albumin, and globulin increased significantly with 0.05% DMPT supplementation （P〈0.05） and serum total protein, globulin and serum aspartate aminotransferase increased significantly with 0.2% DMPT supplementation （P〈0.05）. Meanwhile, liver and muscle protein levels and protease activity in the digestive track increased significantly as dietary DMPT supplementation increased between 0.02% and 0.5% （P〈0.05）. The liver somatic index increased significantly with 0.05% DMPT supplementation. DMPT supplementation had no effect on muscle crude lipid, ash, amino acid profile, or serum alanine transaminase （P〉0.05）. Our results suggest that growth performance and protein levels were improved by dietary DMPT supplementation in taimen and 0.02%-0.5% DMPT supplementation can increase the protein storage in muscle and liver.

关 键 词：哲罗鱼 DMPT 生长性能 肌肉成分 蛋白质代谢 

分 类 号：S963[农业科学—水产养殖]