不同生长基质对骨骼肌成肌干细胞分化融合的影响及初步分析  

Effects of Differentiation of Skeletal Myoblast on Different Growth Substrate in vitro and Preliminary Analysis

作　　者：李晓雨[1] 高颖娜[1] 汤维芳[1] 李孟[1] 郑宏良[1] 

机构地区：[1]第二军医大学附属长海医院耳鼻咽喉头颈外科,上海200433

出　　处：《医学研究杂志》2014年第5期23-25,共3页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81100724)

摘　　要：目的探讨骨骼肌成肌干细胞C2C12在体外分化为肌管的最佳生长条件。方法把C2C12细胞接种于基质胶(matrigel)、Ⅰ型胶原(collagenⅠ)、多聚赖氨酸(PLL)预包被的24孔板,观察细胞生长情况并刺激分化5天后进行免疫荧光染色,分别计算肌球蛋白(myosin)阳性肌管的融合率。结果基质胶组和多聚赖氨酸组肌管融合率明显高于Ⅰ型胶原组(P<0.01),但是多聚赖氨酸组肌管相对较小且多核肌管数(≥3个核)相对较少。结论在3种基质中,基质胶是促进C2C12细胞分化融合的最佳基质,对于C2C12成肌提供了可靠保证。Objective To investigate optimal growth condition for the diferentiation of C2C12 ceils to myotubes on three different growth substrate in vitro. Methods The myoblast cells were resuspended in 24 - well culture plates which was pre - coated with matri- gel, and poly -lysine. The growth and differentiation of C2C12 were observed, then the cells were examined by myotubes marker Myosin with immunocytofluorescence 5 clays later,and myotubes fusion rate was calculated, respectively. Results The myotubes fusion rates of C2C12 cells in matrigel group and polylysine group was significantly higher than group collagen type - I （P 〈 0.01 ）. However, the myotubes and multinucleated myotubcs number （ ≥ 3 nuclei） was relatively small in polylysine group. Conclusion In three kinds of matrix, Matrigel is the best growth substrate to promote the differentiation and fusion of C2C12 cell and guarantee a reliable myogenesis.

关 键 词：生长基质 骨骼肌成肌干细胞 肌管生成 

分 类 号：R685[医药卫生—骨科学]