乳源抗菌-免疫调节融合肽对卵巢癌细胞增殖、凋亡的影响  

Effect of antibacterial-immunomodulating fusion peptide on proliferation and apoptosis

作　　者：唐宜桂[1] 李小勤[1] 刘琛[1] 雷婷[1] 秦宜德[1] 

机构地区：[1]安徽医科大学生物化学与分子生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2014年第6期715-720,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30872992)

摘　　要：目的研究乳源抗菌-免疫调节融合肽(AIFP)对卵巢癌细胞增殖、凋亡的影响。方法采用MTT法测定AIFP对卵巢癌细胞株SKOV3细胞和原代培养的卵巢癌细胞增殖的影响;流式细胞术测定AIFP对SKOV3细胞和原代卵巢癌细胞凋亡、周期的影响;并用RT-PCR检测AIFP对SKOV3细胞及原代癌细胞凋亡和周期相关基因表达水平的影响,以探讨其可能机制。结果 MTT法显示,AIFP对卵巢癌细胞的增殖具有抑制作用,且具有时间和剂量依赖性,与阴性对照组比较差异有统计学意义(P<0.05,P<0.01)。流式细胞术检测结果显示,AIFP诱导卵巢癌细胞的凋亡,具有剂量和时间依赖性,与阴性对照组比较差异有统计学意义(P<0.05,P<0.01)。RT-PCR结果表明,AIFP可以影响细胞凋亡和细胞周期相关基因(Akt、CDC25C和cyclinB1)的表达。结论 AIFP上调卵巢癌细胞凋亡和周期相关基因Akt、CDC25C和cyclinB1的表达,对卵巢癌细胞株SKOV3具有抑制增殖和促进凋亡的作用。Objective To study the effect of immunomodulating fusion peptide （ AIFP） on human ovarian cancer cell proliferation and apoptosis. Methods The ovarian cancer cell proliferation of both the SKOV3 and primary treading with AIFP in vitro were assayed by MTT method. Apoptotic ratios of these cells were measured by flow cy-tometry（FCM） in vitro. The mRNAs of genes （Akt, CDC25C and cyclinB1） related to cell apoptotis and prolifera-tion were assayed by RT-PCR. Results Compared with the control group, AIFP had significant effect on inhibiting cell proliferation and promoted its apoptosis （ P〈0. 05 , P〈0. 01 ） , which were similar in primary ovarian cancer cells （P〈0. 05,P 〈0. 01）. By RT-PCR analysis, AIFP could significantly decrease the expressions of genes （Akt,CDC25C and cyclinB1） related to cell apoptosis and proliferation in SKOV3 cell line. Conclusion AIFP can decrease the proliferation of human ovarian cancer cell and induce their apoptosis. AIFP can down-regulate Akt,CDC25C and cyclinB1 genes expressions, which may affect ovarian cancer cell proliferation and apoptosis.

关 键 词：AIFP SKOV3 增殖 凋亡 

分 类 号：R392.11[医药卫生—免疫学] R392.12[医药卫生—基础医学]