缬沙坦对人冠脉内皮细胞氧化应激过程中gp91^(Phox)的影响  

Effect on gp91^(Phox) human coronary artery endothelial cells in the process of oxidative stress by valsartan

作　　者：王成[1,2] 韩卫星[1] 吴继军[1] 刘超[3] 刘晓颖[4] 

机构地区：[1]安徽医科大学第一附属医院心血管内科,合肥230022 [2]安徽医科大学第一临床学院诊断学教研室,合肥230032 [3]安徽医科大学基础医学院组织与胚胎学教研室,合肥230032 [4]安徽医科大学生命科学院细胞生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2014年第6期739-742,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81070210)

摘　　要：目的研究缬沙坦对人冠脉内皮细胞(HCAEC)氧化应激过程中gp91Phox的影响。方法对照组:体外贴壁培养HCAEC,不进行其他干预;实验组:相同培养条件下加入缬沙坦(10μmol/L)培养24 h。应用Western blot法和细胞免疫荧光法测定两组细胞中gp91Phox的水平,对两组结果进行统计分析并比较。结果实验组HCAEC中gp91Phox的水平明显低于对照组,差异有统计学意义(P<0.01)。结论缬沙坦可以显著减少HCAEC中尼克酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚基gp91Phox的表达,从而降低氧化应激水平。Objective To study the effect on human coronary artery endothelial cells （HCAEC） gp91Phox in the process of oxidative stress by valsartan. Methods The control group： adherent culture HCAEC in vitro, no other intervention; the experimental group： under the same conditions with valsartan （ 10 μmol/L） train for 24 hours. U- sing Western blot and cell immunofluorescence measure gp91Phox levels in two groups of cells. The results were sta- tistically analyzed and compared in both groups. Results The experimental group gp91Phox level significantly lower than the control group in HCAEC （P 〈 0.01 ） , the difference was statistically significant. Conclusion Valsartan can significantly decrease the nicotinamide adenine dinucleotide phosphate（NADPH） oxidase subunit gp91Phox ex- pression in HCAEC, thus reduce oxidative stress level.

关 键 词：缬沙坦 人冠脉内皮细胞 氧化应激 gp91Phox 

分 类 号：R541.4[医药卫生—心血管疾病] R972.4[医药卫生—内科学]