天麻素预处理减轻大鼠心肌缺血再灌注损伤的可能机制  

The possible mechanism of gastrodin precondition on attenuating reaction of myocardial ischemia reperfusion injury in rats

作　　者：位凯[1] 王飞[1] 张瑾[1] 李珍[1] 沈兵[1] 王烈成[1] 孔德虎[1] 胡金兰[1] 

机构地区：[1]安徽医科大学基础医学院生理教研室,合肥230032

出　　处：《安徽医科大学学报》2014年第6期756-758,841,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30900420);安徽省杰出青年基金资助(编号:1108085J11);校级优秀青年教师后备人选基金资助(编号:2009A208)

摘　　要：目的探讨天麻素预处理减轻大鼠心肌缺血再灌注损伤(MIRI)作用的可能机制。方法健康SD大鼠60只,随机分为假手术组,MIRI组,天麻素低、中、高剂量组(0.1、0.2、0.4 g/kg),采用结扎大鼠左冠状动脉前降支30 min,再灌注120 min的方法制备MIRI模型,分别测定血清及心肌组织中肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)的变化以及心肌组织中肌浆网Ca2+-ATP酶(SERCA)的变化。结果 0.2、0.4 g/kg天麻素预处理后,血清及心肌组织中TNF-α和IL-6的含量均降低(P<0.05),心肌组织中SERCA的含量均升高(P<0.01)。结论天麻素预处理减轻大鼠MIRI的机制可能与减少炎症因子TNF-α和IL-6的释放以及增加SERCA含量有关。Objective To observe the possible mechanism of Gastrodin precondition on attenuating reaction of myo-cardial ischemia reperfusion injury （ MIRI） in rats. Methods Sixty SD rats were randomized into five groups such as Sham group, MIRI group and Gastrodin low-dose（0. 1 g/kg）,middle-dose （0. 2 g/kg） and high-dose （0. 4 g/kg） groups. Then the rats mode of myocardial I/R injury were prepared by closing the left anterior descending cor-onary artery for 30 mins and then releasing it for 2 hrs under anesthesia. The changes of tumor necrosis factor α（TNF-α） and interleukin-6 （IL-6） in blood serum and myocardial tissue were determined, and sareoplasmic retic-ulum Ca2＋-ATP （ SERCA） was determined in myocardial tissue. Results Compared with MIRI group, the TNF-αand IL-6 of blood serum and myocardial tissue were lower （ P〈0. 05 ） and the concentration of SERCA of myocar-dial tissue was higher （ P〈0. 01 ） in Gastrodin group of middle and high concentrations. Conclusion Gastrodin preconditioning can attenuate myocardium ischemia reperfusion injury in rats which maybe related to its anti-inflam-mation function and its increase of SERCA.

关 键 词：天麻素 缺血再灌注损伤 肿瘤坏死因子 白介素-6 心肌肌浆网Ca2+-ATP酶 

分 类 号：R33-33[医药卫生—人体生理学] R932[医药卫生—基础医学]