葛根总黄酮对去势大鼠骨质疏松的保护作用  

Protective effect of total flavones of Pueraria lobata on osteoporosis in ovariectomized rats

作　　者：陈冠儒[1] 陈飞虎[1] 葛金芳[1] 王晓宇[1] 詹侠[1] 朱娇[1] 武超[1] 

机构地区：[1]安徽医科大学药学院基础与临床药理教研室,合肥230032

出　　处：《安徽医科大学学报》2014年第6期759-764,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技攻关计划(编号:12010402090)

摘　　要：目的研究葛根总黄酮对去势大鼠骨质疏松的保护作用。方法 10周龄SPF级SD雌性大鼠随机分为正常组、假手术组、去势模型组、替勃龙组(1.25 mg/kg)和葛根总黄酮(50、100、200 mg/kg)给药组。各给药组按上述剂量灌胃给药,每天1次,持续12周。12周后,双能X射线骨密度仪测定各组大鼠右股骨骨密度(BMD)和骨矿物含量(BMC),三点弯曲实验测左股骨生物力学的性能,左胫骨HE染色做组织形态学分析。ELISA法测尿液的羟脯氨酸(HOP)、血清骨特异性碱性磷酸酶(BALP)、卵泡生成素(FSH)、黄体生成素(LH)和雌激素(E2)水平,RT-PCR法检测下丘脑促性腺激素释放激素(GnRH)mRNA的水平。结果葛根总黄酮(100、200 mg/kg)可显著增加去势大鼠右股骨BMD、左股骨生物力学性能,上调下丘脑GnRH mRNA表达,并可降低尿液HOP、血清BALP、FSH和LH的水平,减少骨吸收。结论葛根总黄酮对去势大鼠的骨质疏松有保护和预防性治疗的作用。Objective To investigate the protective activity of total flavones of Pueraria lobata on osteoporosis in ovariectomized rats. Methods 10-week-old SPF female SD rats were randomly assigned to groups including：nor- mal control group, sham ovariectomy group, ovariectomy group, Tibolone Tablets 1.25 mg/kg group and total fla- vones of Pueraria lobata 50, 100, 200 mg/kg groups. All experiment substances were administered for 12 weeks o- rally according to the designed dosages. At the end of 12 weeks treatment, bone minernal density （BMD） , bone minernal content （BMC） of the right femur and bone breaking strength of the left femur were evaluated by dual-en- ery X-ray absorptiometry and three-point bending test, respectively. Histomorphology of the left tibia was analyzed after haematoxylin and eosin staining. Urine hydroxyproline （HOP）, serum bone specific alkaline phosphatase （ BALP）, follicle-stimulating hormone （ FSH）, luteotropic hormone （LH） and estrogen （E2） were mersured by ELISA kits. The mRNA level of hypothalamus gonadotropin-releasing hormone （GnRH） was evaluated by RT- PCR. Results Total flavones of Pueraria lobata （ 100,200 mg/kg） distinctly improved BMD of the right femur, bone biomechanical properties of the right femur, up-regulated mRNA expression of the hypothalamus GnRH and decreased urine HOP, serum BALP, FSH, LH levels and decreased bone absorption. Conclusion Results of cur- rent study demonstrat that treatment of total flavones of Pueraria lobata may protect and preventively treat osteoporo- sis in ovariectomized rats.

关 键 词：葛根总黄酮 去势 骨质疏松 骨密度 

分 类 号：R965.2[医药卫生—药理学]