白芍总苷对糖尿病大鼠肾组织中内质网应激的影响  

Effect of total glucosides of paeony on endoplasmic reticulum stress in the kidney from diabetic rats

作　　者：武晓旭 章超群[1] 许坤[1] 徐兴欣[1] 吴永贵[1] 

机构地区：[1]安徽医科大学第一附属医院肾脏内科,合肥230022

出　　处：《安徽医科大学学报》2014年第6期768-772,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81270813;81374034);教育部高等学校博士学科点专项科研基金(编号:20093420110003)

摘　　要：目的探讨白芍总苷(TGP)对糖尿病肾脏组织中内质网应激(ERS)的影响。方法 50只大鼠随机分为对照组、模型组和TGP给药组[50、100、200 mg/(kg·d)灌胃]。用链脲佐菌素(STZ)诱导大鼠制备糖尿病模型,8周后留取适量肾组织用免疫组织化学法检测葡萄糖调节蛋白78(GRP78)、磷酸化的蛋白激酶样内质网激酶(p-PERK)及磷酸化的真核细胞翻译起始因子2α(p-eIF2α)蛋白的表达。结果各TGP给药组大鼠尿白蛋白排泄率(UAER)较模型组明显减少(P<0.01),模型组大鼠UAER水平明显高于对照组(P<0.01)。与对照组相比,模型组大鼠p-PERK在肾小管-间质中的表达以及GRP78、p-eIF2α在肾小球、肾小管-间质中的表达均显著增加(P<0.01)。与模型组相比,TGP各给药组大鼠GRP78、p-PERK在肾小管-间质中的表达以及p-eIF2α在肾小球、肾小管-间质中的表达明显降低(P<0.01)。结论糖尿病大鼠肾脏中ERS反应明显,TGP对糖尿病肾病大鼠的肾脏保护可能与抑制ERS反应有关。Objective To study the effect of total glucosides of paeony （TGP） on endoplasmic reticulum stress in the kidney from experimental diabetic rats. Methods Rats were randomly divided into three groups：control group, model group and TGP treated groups. Diabetes rat model was induced with streptozotocin. In TGP group, TGP 50, 100,200 rag/（ kg ·d） were orally administered once a day for 8 weeks. The expressions of GRP78, p-PERK and p- eIF2α were determined by immunostaining in the kidney. Results The urinary albumin excretion rate（UAER） in model group was significantly higher than that in TGP treatment group. Analysis of the immunostaining showed the expression of GRP78, p-PERK and p-eIF2α was significantly increased compared with control rats in the kidney. TGP treatment with 50,100 and 200 mg/（ kg· d） could markedly reduce GRP78 and p-PERK expression in the tu- bulointerstitium, p-eIF2α expression in the glomeruli and tubulointerstitium. Conclusion The TGP protection of re- nal tissue in diabetic rats may be associated with inhibition of endoplasmie reticulum stress.

关 键 词：糖尿病肾病 内质网应激 白芍总苷 

分 类 号：R587.24[医药卫生—内分泌] R285.5[医药卫生—内科学]