慢性阻塞性肺疾病、肺炎和肺癌患者血清HMGB1水平及临床意义  

The elevated level of HMGB1 in serum and its clinical role in patients with COPD,pneumonia and lung cancer

作　　者：崔正森[1,2] 李菡[1,2] 姜宝珍[1,2] 张志红[1,2] 

机构地区：[1]安徽医科大学第一附属医院老年呼吸内科 [2]安徽医科大学呼吸病研究所,合肥230022

出　　处：《安徽医科大学学报》2014年第6期812-815,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省归国留学人员择优项目资助(编号:皖人社秘[2009]330号);安徽医科大学中青年学术骨干基金(编号:校人字[2009]38号);安徽省自然科学基金(编号:1208085MH167)

摘　　要：目的探讨血清高迁移率族蛋白B1(HMGB1)在慢性阻塞性肺疾病急性加重期(AECOPD)、肺炎、肺癌患者中的表达水平及意义。方法按照入组标准纳入AECOPD患者40例,肺炎患者40例,非小细胞肺癌(NSCLC)患者30例,健康对照者30例;抽取AECOPD、肺炎、肺癌患者及健康对照者全血,检测HMGB1(ELISA法)水平,外周血白细胞(WBC)计数、C-反应蛋白(CRP)水平以及肺癌标志物如癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、细胞角质蛋白19片段(CYFRA21-1)等。结果 AECOPD、肺炎、肺癌患者血清HMGB1浓度水平高于健康对照者(P<0.01),直线相关分析显示AECOPD及肺炎患者HMGB1水平与WBC(P=0.008,P=0.002)及CRP(P=0.001,P=0.001)均呈正相关,NSCLC患者血清HMGB1水平与CEA和CYFRA21-1(P=0.036,P=0.008)呈正相关。结论血清HMGB1水平在AECOPD、肺炎、肺癌患者明显升高,提示其可能在呼吸系统炎症性疾病及肺癌发病机制中起重要作用,并对疾病的严重程度有一定的提示。Objective To explore the clinical significance of high mobility group box 1 （ HMGB1 ） level in acute exacerbation chronic obstructive pulmonary disease（ AECOPD）, pneumonia and lung cancer patients. Methods Based on the criteria, 40 AECOPD patients, 40 pneumonia patients, and 30 non-small cell lung cancer patients were recruited in this clinical study, meanwhile 30 healthy volunteers were recruited as controls. The peripheral blood samples were collected before any treatment was performed on them and the concentrations of HMGB1 were detected by ELISA. Specific bio-markers of lung cancer such as carcinoma embryonic antigen（CEA） , neuron-spe- cific enolase（NSE） and cytokeratin 19 fragment（CYFRA21-1 ） were measured in cancer patients and healthy vol- unteers. Other clinical data such as white blood cell（WBC） count and C reactive protein（CRP） were also collect- ed and analyzed. Results The serum concentrations of HMGB1 in AECOPD, pneumonia and lung cancer patients were higher than those in the control group respectively（ P 〈 0. 01 ）. In addition, HMGB1 had a positive correlation- ship with the leukocyte count （P = 0. 008, P = 0. 002） and CRP（ P = 0. 001, P = 0. 001 ） level in AECOPD and pneumonia patients; in lung cancer group, HMGB1 was correlated positively with the tumor bio-markers （P = 0. 036 ,P = 0. 008）. Conclusion The serum level of HMGB1 is significantly high in patients of AECOPD, pneu- monia and lung cancer, which indicates that HMGB1 may play a very important role in the development and severity of inflammation and tumor in respiratory system.

关 键 词：高迁移率族蛋白B1 慢性阻塞性肺疾病急性加重期 肺炎 肺癌 

分 类 号：R563[医药卫生—呼吸系统] R734[医药卫生—内科学]