高效液相色谱法测定人血浆拉莫三嗪血药浓度  

Determination of human plasma concentration of lamotrigine with high performance liquid chromatography

作　　者：戴柔丽[1] 朱余兵[2] 洪明岳[1] 

机构地区：[1]南京医科大学附属明基医院药剂部,210021 [2]南京医科大学附属南京第一医院药剂部

出　　处：《江苏医药》2014年第9期1024-1026,共3页Jiangsu Medical Journal

基　　金：南京医科大学科技发展基金面上项目(2011NJMU072)

摘　　要：目的建立测定拉莫三嗪血药浓度的高效液相色谱法(HPLC)。方法色谱柱为Kromasil C18柱(4.6mm×150mm,5μm),甲醇:5mmol/L磷酸二氢钾溶液=45∶55(v/v)。柱温30℃,流速1.0ml/min,检测波长225nm,进样量20μl。以利奈唑胺为内标,测定癫痫患者给药后的血拉莫三嗪浓度。结果拉莫三嗪血药浓度在0.5-50μg/ml的范围内线性关系良好,回归方程为Y=0.1054C+0.2267(r=0.9998)。该方法回收率为97.47%-103.21%,提取回收率为97.19%-104.33%,日内和日间精密度分别为2.67%-5.38%和2.64%-4.45%。结论该方法简单易行,准确度高,可重复性强,适用于拉莫三嗪的临床药物监测和药动学研究。Objective To establish the HPLC method for the determination of lamotrigine in human plasma. Methods The analysis was conducted on a Kromasil C18 （4.6 mm×150 mm, 5μm） with methnol：5 mmol/L biphosphate sodium=45 ： 55 （v/v） in the mobile phase. The flow rate was 1.0 ml/min and the column temperature was 30℃. The detection wavelength was set at 225 nm, and injection volume was 20 /11. Linezolid was used as the internal standard for detecting plasma concentration of lamotrigine. Results When plasma concentration of lamotrigine was in the range of 0. 5-50μg/ml,the regression equation was as follows. Y=0. 1054C＋0. 2267（r=0. 9998） with a good linear relationship. The method recovery ratio was 97.47 % to 103.21%, the extraction recovery ratio was 97.19% to 104. 33%. The within-day precision and day to day precision were 2.67% to 5.38% and 2.64 % to 4. 45%, respectively. Conclusion The method mentioned above is simple, accurate and reproducible, which can be used for testing plasma concentration of lamotrigine and pharmacokinetic study.

关 键 词：高效液相色谱法 拉莫三嗪 血药浓度 

分 类 号：R961[医药卫生—药理学]