镍冶炼烟尘诱导NIH/3T3细胞氧化损伤作用的研究  

Oxidative damage of NIH/3T3 cells induced by nickel smelting fumes

作　　者：巴婧翀 王玥[1] 王声远[1] 焦冬[1] 吴永会[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,150086

出　　处：《中华劳动卫生职业病杂志》2014年第5期348-351,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目（81372965）

摘　　要：目的 研究镍冶炼烟尘对NIH/3T3细胞的毒性作用和氧化损伤作用.方法 使用采自我国某镍冶炼厂的镍冶炼烟尘,用PBS配制浓度为0、6.25、12.50、25.00、50.00和100.00μg/ml的镍冶炼烟尘悬液,对NIH/3T3细胞染毒6h,进行CCK-8、中性红摄取（neutral red uptake,NRU）和乳酸脱氢酶（LDH）泄漏实验观察镍冶炼烟尘的细胞毒性;检测过氧化氢酶（CAT）活力、超氧化物歧化酶（SOD）抑制百分率及丙二醛（MDA）含量,评价细胞氧化损伤程度.结果 染毒组NIH/3T3细胞相对存活率随着镍冶炼烟尘浓度的增加而降低,CCK-8实验100 μg/ml染毒剂量组细胞生长抑制率达到86％,与对照组的差异有统计学意义（P＜0.05）;LDH活力随染毒浓度的增加而增加,12.50、25.00、50.00、100.00 μg/ml染毒剂量组LDH活力明显高于对照组,差异有统计学意义（P＜0.05）;与对照组比较,25.00、50.00和100.00μg/ml染毒剂量组细胞CAT活力明显降低,差异有统计学意义（P＜0.05）;与对照组比较,随着染毒剂量增加,SOD抑制百分率明显上升,MDA含量增加,差异均有统计学意义（P＜0.05）.结论 NIH/3T3细胞暴露于镍冶炼烟尘6h后能够造成抗氧化系统损伤,导致细胞死亡.Objective To investigate the cytotoxicity and oxidative damage on NIH/3T3 cells induced by nickel smelting fume.Methods NIH/3T3 cells were treated with nickel smelting fume collected from a nickel smelting factory in China with doses of 0,6.25,12.50,25.00,50.00,and 100.00 μg/ml for 6 h.Dose-dependent cytotoxicity in cells were assessed by Cell Counting Kit-8 （CCK-8）,natural red uptake assay,and lactate dehydrogenase （LDH） leakage assay,and the level of oxidative damage was assessed based on the activity of catalase （CAT）,percentage inhibition of superoxide dismutase （SOD）,and content of malonaldehyde （MDA）.Results The relative survival of NIH/3T3 cells decreased with the increase in the dose of nickel smelting fume.In the CCK-8 assay,the group with 100 μg/ml nickel smelting fume showed a cell growth inhibition rate of 86％,with a significant difference compared with the control group （P＜0.05）.LDH activity increased with increasing dose of nickel smelting fume：the groups of 12.50,25,50,and 100 μg/ml nickel smelting fume all showed increased LDH activities as compared with the control group （P＜0.05）.The activities of CAT were significantly reduced in groups of 25,50,and 100 μg/ml nickel smelting fume as compared with that of the control group （P＜0.05）.As the dose of nickel smelting fume increased,the percentage inhibition of SOD and the content of MDA increased,with significant differences compared with the control group （P＜0.05）.Conclusion Oxidative damage may be induced in NIH/3T3 cells after 6 h of exposure to nickel smelting fume,which leads to cell death.

关 键 词：镍 粉尘 细胞毒性 丙二醛 过氧化酶 

分 类 号：R135.1[医药卫生—劳动卫生]