血液中N-甲基氨甲酰血红蛋白加合物作为二甲基甲酰胺接触生物标志的研究  

Study of N-methylcarbamoylated hamoglobin in blood of being an exposure biomarker of N, N-dimethylformamide

作　　者：陈伟国[1] 阮征[1] 徐承敏[1] 钱亚玲[1] 

机构地区：[1]浙江省医学科学院卫生学研究所,杭州310013

出　　处：《中华劳动卫生职业病杂志》2014年第5期361-364,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的 探讨血液中N-甲基氨甲酰血红蛋白加合物（N-methylcarbamoylated haemoglobin,NMHb）作为N,N-二甲基甲酰胺（N,N-dimethylformamide,DMF）接触生物标志的可行性.方法 选择某合成革厂72名DMF作业工人为接触组,选择不接触DMF的某食品厂12名工人为对照组.接触组中选择有代表性45名工人进行工作场所空气长时间个体采样,同时问卷调查,并收集尿液和血液,分别用于测定尿甲基甲酰胺（N-methylformamide,NMF）、尿肌酐（Cr）和血液中NMHb.空气中的DMF和尿NMF用气相色谱进行测定,血液中的NMHb经埃德曼降解法降解为3-甲基-5-异丙基乙内酰脲（3-methyl-5-isopropylhydantoin,MVH）后,通过GC/MS进行测定.结果 工作场所空气中DMF浓度和班末尿NMF含量均与血液中NMHb含量相关,回归方程式分别为lgNMHb（nmol/g globin）=0.32×Lg DMF（mg/m3）＋1.8（r=0.60,P＜0.01）,lgNMHb（nmol/g globin）=0.47×lg NMF（mg/g Cr）＋ 1.4（r=0.56,P＜0.01）.结论 NMHb可作为反映DMF接触工人长期接触的生物标志物.Objective To assess the value of blood N-methylcarbamoylated haemoglobin （NMHb） as a biomarker of N,N-dimethylformamide （DMF） exposure.Methods Seventy-two DMF processing workers in a synthetic leather factory were included in the DMF exposure group,and 12 workers in a food factory without exposure to DMF were included in the control group.Long-time individual sampling in workplace was performed among 45 workers in the exposure group,accompanied by a questionnaire survey.Blood and urine were collected for the determination of urinary N-methylformamide （NMF）,urinary creatinine,and blood NMHb.Air DMF and urinary NMF were determined by gas chromatography.NMHb in blood was degraded to 3-methyl-5-isopropylhydantoin by Edman degradation before it could be determined by gas chromatography/mass spectrometry.Results Air DMF in workplace and NMF in post-shift urine were both correlated with NMHb in blood,and the respective regression equations were LgN MHb （nmol/g globin） =0.32×LgDMF （mg/m3） ＋ 1.8 （r=0.60,P＜0.005）,and LgNMHb （nmol/g globin）=0.47×LgNMF （mg/g Cr） ＋ 1.4 （r=0.56,P＜0.005）.Conclusion NMHb can be used as a biomarker of long-term exposure to DMF.

关 键 词：二甲基甲酰胺(DMF) 生物标志物 N-甲基氨甲酰血红蛋白加合物(NMHb) 甲基甲酰胺(NMF) 

分 类 号：R135[医药卫生—劳动卫生]