亚慢性染毒纳米碳酸钙大鼠组织病理学研究  

The histopathological change in organs of rats sub-chronic exposed to nano-calcium carbonate

作　　者：胡博骅 刘卫花[1] 冯涓[1] 钱怡[1] 仇玉兰[1] 唐仕川[2] 王煜倩[2] 郑金平[1] 

机构地区：[1]山西医科大学公共卫生学院毒理学教研室,山西太原030001 [2]北京市劳动保护科学研究所

出　　处：《毒理学杂志》2014年第2期91-94,共4页Journal of Toxicology

基　　金：北京市科技创新工程项目(PXM2012_178304_000007);山西省优势与特色重点学科建设经费

摘　　要：目的通过观察亚慢性染毒纳米碳酸钙大鼠器官组织病理学变化,初步探讨纳米碳酸钙的毒性和可能靶器官。方法选取健康SD大鼠100只,随机分为对照组、微米碳酸钙组(200 mg/kg)、纳米碳酸钙组(12.5、50和200 mg/kg)。滴鼻染毒,每周5次,连续12周。染毒结束24 h后处死大鼠,腹主动脉采血,全自动生化分析仪检测天冬氨酸转氨酶(AST)及丙氨酸转氨酶(ALT)活性和肌酐(Cr)、尿素氮(BUN)含量,取大鼠心、肝、脾、肺、肾、脑海马组织进行病理观察。结果对照组大鼠各器官均未见异常。微米碳酸钙组大鼠肺泡壁充血水肿,炎性细胞侵润。纳米碳酸钙组大鼠肺泡壁充血水肿,炎性细胞浸润,局部肺不张,部分小血管出现玻璃样变;支气管黏膜萎缩、剥脱,大量炎性细胞浸润。高剂量纳米碳酸钙组大鼠肾小球肿胀,肝细胞脂肪样变。纳米碳酸钙各组大鼠血清ALT活性和BUN含量显著高于对照组(P<0.05),高剂量组大鼠血清BUN含量显著高于微米碳酸钙组(P<0.05)。结论亚慢性染毒纳米碳酸钙可导致大鼠肺、肝、肾组织病理学损伤。Objective To analysis the toxicity and potential target organs of nano-calcium carbonate through observing the pathological changes of rats sub-chronic exposed to nano-calcium carbonate. Methods 100 healthy SD rats were randomly divided into control group, miCron-calcium carbonate group （200 mg/kg） , and nano-calcium carbonate groups （12.5, 50, 200 mg/kg） All rats were given test substances by intranasal method 5 times a week for 12 weeks. Blood plasma was collected by abdominal aortic method. And automatic biochemical analyzer was used to test the content of Aspartate aminotransferase （AST）, Alanine aminotransferase （ALT） activity and Creatinine （ Cr）, Blood urea nitrogen （BUN） in rat＇ s serum. Observing the pathological change of the rat＇ s heart, liver, spleen, lung, kidney, brain hippocampus after 24 hours of last exposure of. test substances. Results All organs of the rats in the blank group showed no abnormality. The rats in micron calcium carbonate group suffered from alveolar wall hyperemia and edema, and inflammatory cells infiltration. The rats in nano-calcium carbonate groups suffered from alveolar wall hyperemia and edema, inflammatory cells infiltration, local pulmonary atelectasis, and hyaline change in part of the small blood vessels; as well as bronchial mucosa atrophy and denudation, a large number of inflammatory cells infiltration. Glomerulus swelling and hepatocellular lipoid degeneratiaon were found in high-dose nano-calcium carbonate group rats. All nano-calcium carbonate groups＇ serum ALT activity and BUN content were significantly higher than those in the control group （P 〈 0. 05 ） , the content of BUN in serum of rats in high dose group was significantly higher than that of micron calcium carbonate group （P 〈 0.05）. Conclusion The sub-chronic exposure to nano-calcium carbonate can lead to histopathology lesions in rats＂ lung, liver and kidney.

关 键 词：纳米碳酸钙 病理学 亚慢性染毒 

分 类 号：R114[医药卫生—卫生毒理学]