变频振动在模拟微重力环境下对成骨细胞增殖和分化的影响  

The effect of variable-frequency vibration on the proliferation and differentiation of osteoblasts in simulated microgravity environment

作　　者：陶飞飞[1] 吴继功[2] 马华松[2] 宋淑军[3] 刘俊丽[3] 邵水霖[2] 张乐乐[2] 陶有平[2] 高博[2] 李海侠[2] 

机构地区：[1]安徽医科大学,合肥230032 [2]解放军306医院骨科,北京100101 [3]解放军306医院特种医学实验研究中心,北京100101

出　　处：《中国骨质疏松杂志》2014年第5期504-507,512,共5页Chinese Journal of Osteoporosis

基　　金：全军医药卫生"十二五"科研课题(CWS12J140)

摘　　要：目的：观察变频振动在模拟微重力环境下对体外培养成骨细胞增殖和分化的影响。方法利用沿水平轴连续回转（30 r/min）细胞培养系统模拟微重力环境，应用电磁振动台将振动强度为0.5 g（g为加速度）在不同频率45 Hz、90 Hz、变频（5～90 Hz）振动应力作用于新生24 h大鼠颅盖骨成骨细胞，分别使用MTT比色法和碱性磷酸酶（ ALP）活性测定了解成骨细胞的增殖和分化情况。结果模拟微重力环境可以抑制新生24 h 大鼠颅盖骨成骨细胞的增殖和分化功能；90 Hz 和5～90 Hz变频振动促进模拟微重力环境成骨细胞的增殖功能（p＜0.01）；45 Hz（p＜0.05）和5～90 Hz（p＜0.01）变频振动刺激对模拟微重力环境成骨细胞的分化功能具有一定的保护作用。结论在模拟微重力环境下机械振动对成骨细胞的增殖和分化功能具有保护作用，这为机械振动刺激防治微重力环境下骨丢失提供了理论和实验依据。Objective To investigate the effect of variable-frequency vibration on the proliferation and differentiation of osteoblasts in simulated microgravity environment in vitro.Methods The simulated microgravity environment was created using continuous rotary cell culture system （30 r/min） along with the horizontal axis .The electromagnetic vibration table was used to create the force with the vibration intensity of 0.5 g （ g：the acceleration of gravity ） and 45 Hz, 90 Hz, and variable frequencies （5-90 Hz） vibration.The force was applied to the osteoblasts collected from newborn 24-hour rat cranium .The proliferation and differentiation of osteoblasts were detected using MTT colorimetry and alkaline phosphatase （ALP） activity determination, respectively.Results The simulated microgravity environment could inhibit the proliferation and differentiation of osteoblasts from the newborn 24-hour rat cranium.But with the stimulations of 90 Hz and 5 -90 Hz frequency vibrations in the simulated microgravity environment, the proliferation of osteoblasts was promoted （ P〈0.01 ） .And the stimulations of 45 Hz and 5 -90 Hz frequency vibrations could protect the differentiation of osteoblasts （ P 〈0.05 and P 〈0.01, respectively）.Conclusion In the simulated microgravity environment , the mechanical vibration can protect the proliferation and differentiation of osteoblasts , which provides theoretical and experimental basis for the application of mechanical vibration in the prevention and treatment of osteoporosis in the low gravity environment .

关 键 词：微重力 变频振动 成骨细胞 增殖 碱性磷酸酶 

分 类 号：R318[医药卫生—生物医学工程]