补肾活血方对去势骨质疏松大鼠循环Serotonin表达的影响  

Effect of nourishing the kidney and invigorating the blood recipe on circulating serotonin level in ovariectomized rats

作　　者：梁冬波[1] 王力平[1] 覃智斌[1] 唐福宇[1] 王润生[1] 关威[1] 

机构地区：[1]柳州市中医院,广西柳州545001

出　　处：《中国骨质疏松杂志》2014年第5期508-512,共5页Chinese Journal of Osteoporosis

摘　　要：目的探讨补肾活血方对去势大鼠循环5-羟色胺(Serotonin)表达水平的影响。方法 30只6月龄SD雌性大鼠去卵巢制作骨质疏松模型,另取10只行假手术,建立假手术组。3个月后,卵巢切除的大鼠随机分为三组,然后分别予补肾活血方(中药组),阿仑膦酸钠(西药组)和生理盐水(模型组)治疗,假手术组给予等量生理盐水。经过8周的治疗后处死大鼠,检测左侧股骨骨密度,ELISA检测血清Serotonin、核因子κB受体活化因子配体(RANKL)和骨转换指标P1NP、CTX水平。结果模型组骨密度低于假手术组(P<0.001);中药组和西药组全段股骨骨密度高于模型组(P<0.01和P<0.001)。中药组和西药组血清P1NP低于模型组(P均<0.001),而假手术组、中药组与西药组之间无统计学差异。中药组和西药组血清CTX低于模型组(P均<0.001),高于假手术组(P均<0.001)。中药组血清Serotonin水平低于西药组和模型组(P均<0.001),假手术组与中药组之间无统计学差异。中药组和西药组血清RANKL低于模型组(P均<0.001),假手术组、中药组与西药组之间无统计学差异。结论补肾活血方可能是通过降低去势大鼠循环Serotonin表达水平,进而抑制RANKL表达水平,从而提高去势大鼠骨密度。Objective To investigate the effect of nourishing the kidney and invigorating the blood recipe on the circulating serotonin level in ovariectomized rats.Methods Thirty 6-month female Sprague Dawley rats were ovariectomized to induce osteoporosis, and another 10 rats underwent sham operation to establish sham-operated （ SHAM ） group.After 3 months, the ovariectomized rats were randomly divided into 3 groups： herb group, alendronate group, and OVX group.Rats in herb group were treated with nourishing the kidney and invigorating the blood recipe;while rats in alendronate group were treated with alendronate .Rats in OVX group and SHAM group were treated with normal saline .After 8-week treatment , all the rats were sacrificed .The bone mineral density （BMD） of the left femur was detected.The serum levels of serotonin, RANKL, and bone turnover markers, including P1NP and CTX, were detected using ELISA.Results BMD in OVX group was lower than that in SHAM group （P〈0.001）, while BMD in the intact femur in herb group and alendronate group was higher than that in OVX group （P〈0.01 and P〈0.001）.The serum P1NP level in herb group and alendronate group was significantly lower than that in OVX group （P〈0.001）, while no significant difference among SHAM group, herb group, and alendronate group was observed.The serum CTX level in herb group and alendronate group was lower than that in OVX group （P〈0.001）, while it was higher than that in SHAM group （P〈0.001）. The serum serotonin level in herb group was significantly lower than that in OVX group and alendronate group （P〈0.001）.But no significant difference between SHAM group and herb group was observed.The serum RANKL level in herb group and alendronate group decreased significantly compared with that in OVX group （P〈0.001）, and no significant difference among SHAM group, herb group, and alendronate group was observed.Conclusion Nourishing the kidney and invigorating blood recipe can enhance BMD in OVX rats most likely by redu

关 键 词：中药 去势大鼠 骨质疏松 

分 类 号：R274[医药卫生—中医骨伤科学]