Effect of Temperature on Gene Expression in the Pearl Oyster Pinctada fucata  

作　　者：LIU Wenguang HUANG Xie LIN Jianshi HE Maoxian 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology,Chinese Academy of Sciences

出　　处：《Journal of Ocean University of China》2014年第3期509-515,共7页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (41006090);Joint Program of NSFC-Guangdong (U0831001);the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21)

摘　　要：In this study, we examined the effect of elevated temperature on the expression patterns of genes, i.e., nacrein, irr, n16, n19, and hsp70 in the pearl oyster Pinctada fucata. The experiment was carried out at 4 temperatures, i.e., 20℃(ambient, control), 24, 28℃, and 32℃. The expression levels of target genes in P. fucata were assayed at 0, 6, 24, 48, and 96 h via real-time polymerase chain reaction. Results showed that the expression levels of nacrein and irr had no significant variations among different time points below 28℃, but significantly increased over time at 32℃. The expression levels of n16 and n19 did not change markedly at 20℃. The former increased significantly at 6 h and 24 h while the latter substantially decreased during 6–96 h at 24, 28 and 32℃. Among different temperatures, the level of n16 was significantly lower at 20℃ than at other temperatures during 6–96 h, and the level of n19 significantly varied among different temperatures at 48 h and 96 h. The expression level of hsp70 was significantly higher at 32℃ than at 20, 24 and 28℃ at 24 h. These results demonstrated that elevated temperature impacted the physiological processes of P. fucata and potentially influenced its adaptability to thermal stress.In this study, we examined the effect of elevated temperature on the expression patterns of genes, i.e., nacrein, irr, n16, n19, and hsp70 in the pearl oyster Pinctada fucata. The experiment was carried out at 4 temperatures, i.e., 20℃（ambient, control）, 24, 28℃, and 32℃. The expression levels of target genes in P. fucata were assayed at 0, 6, 24, 48, and 96 h via real-time poly-merase chain reaction. Results showed that the expression levels of nacrein and irr had no significant variations among different time points below 28℃, but significantly increased over time at 32℃. The expression levels of n16 and n19 did not change mark-edly at 20℃. The former increased significantly at 6 h and 24 h while the latter substantially decreased during 6-96 h at 24, 28 and 32℃. Among different temperatures, the level of n16 was significantly lower at 20℃than at other temperatures during 6-96 h, and the level of n19 significantly varied among different temperatures at 48 h and 96 h. The expression level of hsp70 was significantly higher at 32℃than at 20, 24 and 28℃at 24 h. These results demonstrated that elevated temperature impacted the physiological processes of P. fucata and potentially influenced its adaptability to thermal stress.

关 键 词：seawater temperature heat shock protein gene expression pattern Pinctadafucata 

分 类 号：S968.3[农业科学—水产养殖]