超声辅助离子液体液相微萃取-高效液相色谱法分析水样中加替沙星或氟罗沙星  

Determination of Gatifloxacin or Fleroxacin in Water Sample by Ultrasound-Assisted Ionic Liquid LiquidPhase Microextraction Coupled with High Performance Liquid Chromatography

作　　者：张婧[1] 赵文岩[1] 刘富强[1] 王晓黎[1] 

机构地区：[1]内蒙古大学化学化工学院,呼和浩特010021

出　　处：《应用化学》2014年第6期737-741,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目(21167008);内蒙古自治区教育厅重点项目(NJZZ11014)~~

摘　　要：建立了一种采用离子液体1-己基-3-甲基咪唑六氟磷酸（[C6mim][PF6]）为萃取剂,超声辅助离子液体液相微萃取-高效液相色谱法分析水样中加替沙星和氟罗沙星的方法.实验考察了溶液酸度、离子液体用量等因素对萃取的影响.在pH值分别为3.3、2.1的加替沙星和氟罗沙星水样中,加入0.4mL[C6mim][PF6],超声,离心,离子液体相直接用于HPLC进行分析.该方法的线性范围为0.5～ 50 mg/L,测定加替沙星和氟罗沙星的相对标准偏差（n=5）为2.80％和5.93％,二者的检出限分别为0.46、0.97 μg/L,该方法萃取水样中加替沙星的加标回收率为80.5％～ 89.5％,氟罗沙星的加标回收率可达93.3％～99.0％.A method was established for the analysis of gatifloxacin or fleroxacin in water sample by ultrasonic assisted hydrophobic ionic liquid （1-hexyl-3-methylimidazolium hexafluorophosphate,[C6mim] [PF6]）liquid-phase microextraction coupled with high performance liquid chromatography（HPLC）.The pH value of solution,amount of ionic liquid and other factors which might affect the extraction efficiency were researched.To gatifloxacin water sample at pH =3.3 and fleroxacin water sample at pH =2.1 was added 0.4 mL of [C6mim] [PF6],respectively,for ultrasonic extraction.Mter extraction,phase separation was performed by centrifugation.The sediment phase were injected into the HPLC system for analysis.By this method,the linear ranges for analysis are 0.5 ～ 100 μg/L and the relative standard deviations （n =5） for them are 2.80and 5.93,respectively.The limits of detection （3S/N） for gatifloxacin and fleroxacin are 0.46 and 0.97 μg/L,respectively.Under the above mentioned conditions,the extraction recoveries for gatifloxacin or fleroxacin water samples were found to be 80.5％ ～ 89.5％ and 93.3％ ～ 99.0％,respectively.

关 键 词：加替沙星 氟罗沙星 离子液体 超声波 

分 类 号：O658.2[理学—分析化学]