基于COPAS自动分选系统检测线虫线粒体氧化还原状态(英文)  

Evaluation of The Mitochondrial Redox Status in Caenorhabditis elegans:An Assay Based on The COPAS Biosort Flow Cytometer

作　　者：胡明曦 徐涛[1] 陈畅[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101 [2]中国科学院大学,北京100049

出　　处：《生物物理学报》2014年第2期110-116,共7页Acta Biophysica Sinica

基　　金：supported by grants from The"973"Program(2012CB911000,2011CB503900);The National Natural Sciences Foundation of China(31225012,31030023)~~

摘　　要：线粒体是氧化还原信号通路的重要节点,然而,对于线粒体氧化还原变化的原位检测十分困难。作者选择秀丽隐杆线虫为模式生物,使用线粒体定位的氧化还原敏感探针Grx1-roGFP2对线虫进行标记。同时,为了快速比较多个样品间的氧化还原变化,建立了基于COPAS生物自动分选系统的自动定量检测方案。结合氧化还原敏感探针标记的转基因线虫品系,该方法能够快速检测RNA干扰以及线虫生长发育不同时期引起的线粒体氧化还原变化。Mitochondria have emerged as an important node for redox signaling. However, it is difficult to monitor the actual changes in redox status in situ. The authors selected a mitochondria-targeted redox-sensitive probe, Grxl-roGFP2, for expression in the nematode Caenorhabditis elegans. Using this probe, they developed a rapid, automated and quantitative method for examining changes in redox status in multiple samples with the COPAS （complex parametric analyser and sorter） Biosort, a flow cytometer designed to accommodate large objects such as C. elegans. This assay is capable of rapid, in situ monitoring of the mitochondrial redox status of biosensor-transgenic nematodes during nematode development and can be used to analyze the redox changes caused by RNA interference.

关 键 词：氧化还原 秀丽隐杆线虫 线粒体 roGFP COPAS自动分选系统 

分 类 号：Q-3[生物学] Q2