参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织AQP4蛋白及mRNA表达的影响  

Effect of Shenlin Baizhu powder on expression of AQP4 in ulcerative colitis rats with syndrome of dampness stagnancy due to spleen deficiency

作　　者：李姿慧[1] 王键[1] 蔡荣林[2] 孙娟[1] 叶铭钢[1] 

机构地区：[1]安徽中医药大学中医临床学院,安徽省合肥市230038 [2]安徽中医药大学针灸经络研究所,安徽省合肥市230038

出　　处：《世界华人消化杂志》2014年第12期1688-1693,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81173172;81302901;安徽省自然科学基金资助项目;No.1308085QH155;安徽省高等学校自然科学研究重点基金资助项目;No.KJ2011A184~~

摘　　要：目的:探讨参苓白术散对脾虚湿困型溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠组织中水通道蛋白4(aquaporin 4,AQP4)蛋白及mRNA表达的影响.方法:Wistar大鼠40只,随机分为正常对照组、模型对照组、柳氮磺吡啶组、参苓白术散组,每组10只,脾虚湿困型UC大鼠模型的复制采用环境与饮食干预结合三硝基苯磺酸(trinitrobenzene sulfonic acid,TNBS)/乙醇灌肠法,运用免疫组织化学法、荧光定量PCR法检测并比较各组大鼠结肠组织AQP4蛋白及mRNA表达的变化.结果:模型对照组AQP4蛋白及mRNA表达量低于正常对照组(0.764±0.051 vs 0.358±0.033,28.288±1.578 vs 28.296±1.142,P<0.05),参苓白术散组和柳氮磺吡啶组大鼠结肠组织AQP4表达平均光密度及AQP4mRNA表达较模型对照组显著升高,其间差异有统计学意义(0.752±0.059 vs 0.358±0.033,28.103±1.395 vs 28.288±1.578,0.487±0.046vs 0.358±0.033,27.711±1.638 vs 28.288±1.578,P<0.05),参苓白术散组大鼠结肠组织A Q P4表达平均光密度与柳氮磺吡啶组比较差异亦有统计学意义(0.752±0.059 vs 0.487±0.046,28.103±1.395 vs 27.711±1.638,P<0.05).结论:参苓白术散治疗可以明显改善UC大鼠结肠组织中AQP4的表达,促进肠黏膜的修复.AIM： To investigate the effect of Shenlin Baizhupowder on expression of aquaporin 4 （AQP4） in ulcerative colitis （UC） rats with syndrome of dampness stagnancy due to spleen deficiency. METHODS： Forty rats were divided into a normal control group, a model control group, a Shenlin Baizhu group and a sulfasalazine group, with 10 rats in each group. UC was induced in rats by environmental and diet interventions combined with trinitrobenzene sulfonic acid （TNBS） and ethanol. The expression of AQP4 protein and mRNA in colonic tissues was detected by irnmunohistochemistry and fluorescence quantitative PCR, respectively. RESULTS： The expression of AQP4 protein and mRNA in the model control group was significantly lower than that in the normal group （0.764 ±0.051 vs 0.358 ± 0.033, 28.288 ± 1.578 vs 28.296± 1.142, P 〈 0.05）. The expression of AQP4 protein and mRNA in the sulfasalazine group and Shenlin Baizhu group significantly increased and was significantly higher compared with that in the model group （0.752 ± 0.059 vs 0.358 ± 0.033, 28.103 ± 1.395 vs 28.288 ± 1.578, 0.487 ± 0.046 vs 0.358 ± 0.033, 27.711 ± 1.638 vs 28.288 ± 1.578, P 〈 0.05）. There was a significant difference in the expression of AQP4 protein and mRNA between the sulfasalazine group and Shenlin Baizhu group （0.752±0.059 vs 0.487 ± 0.046, 28.103±1.395 vs 27.711 ± 1.638, P 〈 0.05）. CONCLUSION： Shenlin Baizhu powder could significantly improve the expression of AQP4 protein and mRNA in UC and promote the repair of the intestinal mucosa.

关 键 词：参苓白术散 脾虚湿困证 溃疡性结肠炎 水通道蛋白4 

分 类 号：R285.5[医药卫生—中药学]