低浓度尼古丁对大鼠硬腭软组织缺损愈合的影响  

Effect of low concentration of nicotine on soft tissue defect repair of rat hard palate

作　　者：张亚军[1] 杨聪翀[2] 刘来奎[2] 张梦洁[3] 孙应明[3] 

机构地区：[1]安徽医科大学口腔医学院,安徽省合肥市230032 [2]南京医科大学附属口腔医院基础教研室,江苏省南京市210029 [3]解放军101医院口腔科,江苏省无锡市214044

出　　处：《中国组织工程研究》2014年第15期2326-2331,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81272968)~~

摘　　要：背景:最新系列研究发现低浓度尼古丁具有促进血管生成,加速皮肤伤口愈合的作用,但低浓度尼古丁能否在颌面部软组织创伤修复中尤其是口腔黏膜创伤修复中发挥作用目前还不清楚。目的:观察低浓度尼古丁对大鼠硬腭黏膜缺损修复的影响。方法:Wistar大鼠65只,于硬腭第一磨牙近中至第三磨牙远中的黏膜作一直径为3 mm的圆形切创缺损。造模后局部给药,大鼠随机分成低浓度尼古丁+凝胶组、凝胶组、空白对照组,每组动物分别在3,7,10,14 d处死,苏木精-伊红染色观察组织愈合过程,并通过大体标本结合图像测量分析比较各组间伤口愈合差异。结果与结论:修复后3 d各组间愈合情况差异无显著性意义;修复后7,10 d组低浓度尼古丁+凝胶组愈合快于凝胶组和空白对照组(P<0.05);修复后14 d各组创面已基本愈合,各组间差异无显著性意义。提示低浓度尼古丁可能具有促进大鼠硬腭膜缺损修复的作用。BACKGROUND：Low concentration of nicotine promotes the angiogenesis and facilitates the healing of skin wounds. However, the role of low concentration of nicotine on the repair of maxil ofacial soft tissue trauma especial y oral mucosa stil remains unclear OBJECTIVE：To evaluate the effect of low concentration of nicotine on mucosa defect repair of rat hard palate. METHODS：A circular soft tissue defect at 3 mm diameter was produced in the centre of hard palate of 65 Wistar rats. After the operation, animals were randomly divided into low concentration of nicotine with gel group, gel group and control group. Rats were sacrificed at 3, 7, 10 and 14 days post-surgery. The wound healing was detected with hematoxylin-eosin staining and the difference of wound healing in different groups was compared with gross observation and image measurement. RESULTS AND CONCLUSION：There was no significant difference in the wound healing in different groups on day 3 post-surgery. On days 7 and 10, the group of low concentrations of nicotine with gel was faster than gel group and control group （P〈0.05）;the wounds were completely healed on day 14, with no significant difference among the groups. Low concentrations of nicotine may promote the mucosa defects repair of rat hard palate.

关 键 词：组织构建 软组织构建 尼古丁 软组织缺损 凝胶 创面愈合率 硬腭 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]