工程组织及器官的血管化:研究现状与应用进程  

Vascularization for tissue engineering organs: research progress and application prospect

作　　者：郑幸龙[1,2] 向俊西[1,2] 李建辉[2,3] 吕毅[1,2] 

机构地区：[1]西安交通大学第一附属医院肝胆外科,陕西省西安市710061 [2]西安交通大学先进外科技术与工程研究所,陕西省西安市710061 [3]陕西省人民医院肿瘤外科,陕西省西安市710061

出　　处：《中国组织工程研究》2014年第15期2427-2433,共7页Chinese Journal of Tissue Engineering Research

基　　金：高等学校博士学科点专项科研基金(20110201130009);陕西省科学技术研究发展计划国际合作项目(2012KW-39-01)~~

摘　　要：背景:构建有效的血管系统对于三维结构组织内细胞的存活和功能表达均具有决定性作用。因此,寻找一种合适的血管化策略已成为组织工程领域研究的热点和亟待解决的问题。目的:总结并讨论组织工程血管化的理论基础及研究进展。方法:检索PubMed数据库相关文章,检索时间为2003至2013年,检索词为"tissue engineering,vascularization,endothelial cell,scaffold",并限定文章语言种类为英语,再从中筛选出与主题相关的部分文献,进行总结讨论。结果与结论:初检得到124篇文章,按主题相关性对文献进一步筛选,最终纳入41篇文章。血管化一直是组织工程领域关注的焦点和难点,国内外研究过多种血管化的策略,如复合生长因子、细胞共培养、生物反应器动态培养、人工支架或脱细胞支架等,但尚未公认一种有效的血管化策略实现与受体血管的功能性连接和体内细胞的长期存活。应用诱导多能干细胞与其他干细胞共培养,并通过添加生长因子、优化培养条件等方法在体内定向诱导分化,有望成为实现组织工程器官血管化的突破点。BACKGROUND：An efficient blood vessel system has a decisive effect on the survival and function expression of cells in three-dimensional tissues. Therefore it has been a hot research field in tissue engineering to find an appropriate vascularization strategy. OBJECTIVE：To summarize and discuss the theory and research progress in vascularization strategies. METHODS：Literature search was performed in PubMed database for English literatures published from 2003 to 2013. The key words are“tissue engineering, vascularization, endothelial cell, scaffold”in English. Then, the papers were further analyzed and reviewed in line with the theme. RESULTS AND CONCLUSION：A total of 124 papers were searched. At last, 41 papers were selected according to the titles and objectives. Vascularization is the focus and pressing issue in tissue engineering field. There are many vascularization strategies, such as growth factor delivery, cellco-culture, dynamic-culture by bioreactor, scaffolds or decellularized scaffolds. But none of them is recognized as an effective strategy to achieve functional anastomosis with the host and sustain grafts survival for a long time in vivo. It wil be a big breakthrough in the future to co-culture pluripotent stem cells with other stem cells, combine with growth factors and optimize culture conditions for the differentiation in vivo.

关 键 词：组织构建 血管内皮细胞 组织工程 血管化 内皮细胞 血管化策略 共培养 脱细胞支架 诱导多能干细胞 

分 类 号：R318[医药卫生—生物医学工程]