盐度对大菱鲆幼鱼鳃Na^+-K^+-ATPase活力、血清离子浓度和激素水平的影响  

Effects of Salinity on Na^+-K^+-ATPase Activity in Gills,and Concentrations of Ions and Hormones in Serum of Juvenile Turbot(Scophthalmus maximus)

作　　者：曾霖[1,2] 雷霁霖[1,2] 刘滨[2] 洪万树[1] 艾春香[1] 高淳仁[2] 

机构地区：[1]厦门大学海洋与地球学院,厦门361021 [2]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《动物学杂志》2014年第3期407-414,共8页Chinese Journal of Zoology

基　　金：现代农业产业技术体系专项资金(No.CARS-50)

摘　　要：采用饲养试验方法,研究了平均体质量为(7.16±0.07)g的大菱鲆(Scophthalmus maximus)幼鱼分别在盐度12、18、24、30和36下饲养60 d后,其鳃Na+-K+-ATPase活力、血清离子浓度、生长激素(GH)、皮质醇激素(COR)、特定生长率(SGR)和饲料效率(FCE)的变化。结果表明:幼鱼鳃Na+-K+-ATPase活力、血清Na+浓度均随盐度的升高而上升,分别在3.48~8.30 U/mg和169.99~180.00 mmol/L之间,其中12盐度组最低,36盐度组最高。幼鱼血清中K+和Cl-浓度分别在2.20~3.47 mmol/L和136.67~142.00 mmol/L之间,各盐度组之间差异不显著。幼鱼血清中GH和COR浓度分别在0.41~1.66 ng/ml和35.33~76.41 ng/ml之间;其中GH在36盐度组最高,12盐度组最低,而COR在12盐度组最高,36盐度组最低。幼鱼SGR和FCE分别在(1.45~2.00)%/d和1.12%~1.38%之间,与盐度的相关性不显著,两者均为12盐度组最低。由此可见,盐度变化显著影响大菱鲆幼鱼鳃Na+-K+-ATPase活力、血清Na+浓度和激素含量。本研究对大菱鲆养殖生理生态条件分析具有重要参考意义,研究结果可为大菱鲆养殖的盐度选择提供理论依据。The Na ＋ -K ＋ -ATPase activity in gill, concentrations of ions and hormones in serum, and specific growth rate and feed conversion efficiency of juvenile turbots （Seophthalmus maximus） （body weight of 7. 16 ± 0.07 g）, which have been reared at salinitics 12, 18, 24, 30 and 36 for 60 days, were investigated. The results showed that Na＋ -K＋ -ATPase activity and Na＋ concentration were 3.48 -8.30 U/rag and 169.99 - 180.00 mmol/L, respectively, and their levels increased with the rise of water salinity. The highest values were observed at salinity 36, and lowest ones at salinity 12. The Na ＋ -K ＋ -ATPase activity and Na ＋ concentration inthe fish reared at salinity 12 were significantly lower than those in the control （P 〈 0.05）. K ＋ and CI- concentrations were 2.37 - 3.47 mmol/L and 136.67 - 142.00 mmol/L, respectively, both of which had no significant differences between groups （P 〉 0. 05）. Growth hormone and cortisol levels were 0. 41 - 1.66 and 35.33 -76.41 ng/ml, respectively. Growth hormone level was the lowest in the fish reared at salinity 12, and the highest at salinity 36, both of which had significant difference compared to that of the control （P 〈 0. 05） ; while cortisol level was the highest at salinity 12, and the lowest at 36, with no significant difference compared with that of the control （P 〉 0.05）. Specific growth rate and feed conversion efficiency were （ 1.45 - 2.00） %/day and1. 12% - 1.38% , respectively. Although both were the lowest at salinity 12, they were not significantly correlative with salinity. Thus, the results indicate that the changes in salinity could have significant impacts on Na ＋ -K ＋ -ATPase activity in gill, concentrations of Na ＋ , growth hormone and cortisol in serum of juvenile turbots. The results provide important reference for salinity selection in turbot mariculture.

关 键 词：大菱鲆 盐度 Na+-K+-ATPase 血清离子 生长激素 皮质醇激素 

分 类 号：S917.4[农业科学—水产科学]