急性不完全脊髓损伤模型大鼠相关炎症因子的表达  

Expression of inflammatory factors in model rats with acute incomplete spinal cord injury

作　　者：宗少晖[1] 方晔[2] 彭金珍 高太行[2] 

机构地区：[1]广西医科大学第一附属医院脊柱骨病外科,广西壮族自治区南宁市530021 [2]广西医科大学研究生学院,广西壮族自治区南宁市530021

出　　处：《中国组织工程研究》2014年第18期2806-2811,共6页Chinese Journal of Tissue Engineering Research

基　　金：广西教育厅科研基金资助项目(广西高校科研项目201204LX063)~~

摘　　要：背景:研究提示脊髓损伤早期神经元和神经胶质细胞分泌产生的细胞因子可能是造成损伤后继发免疫损伤的重要因素。目的:分析白细胞介素1β、白细胞介素6、白细胞介素17等炎症因子在急性脊髓损伤后炎症反应过程中的作用。方法:雄性成年SD大鼠75只,随机分为对照组和脊髓损伤1,6,24和72 h组。采用改良Allen氏法建立脊髓不完全损伤模型,对照组仅行椎板全切除。在建模之后相应的时间点获取损伤段脊髓、脾脏组织,免疫组织化学染色法检测白细胞介素6和白细胞介素17在损伤段脊髓组织中的分布及表达情况,蛋白免疫印迹法检测p-STAT3在损伤段脊髓组织中的表达变化,采用RT-PCR法检测白细胞介素1βmRNA、白细胞介素6mRNA和白细胞介素17A mRNA在脾脏组织中的表达情况。结果与结论:脊髓损伤后p-STAT3、白细胞介素1β、白细胞介素6和白细胞介素17的表达明显高于对照组(P<0.05)。损伤后炎症因子表达量立即升高,白细胞介素1β和白细胞介素6在损伤后6 h到达高峰,随后开始下降;p-STAT3和白细胞介素17在损伤后24 h到达高峰,随后开始下降,至损伤后72 h表达量仍高于对照组。结果表明损伤早期p-STAT3介导的促炎因子白细胞介素1β和白细胞介素6表达增加,提示可能导致损伤区域炎症级联放大,诱导继发性脊髓损伤;促炎因子白细胞介素17的异常升高,提示可能在继发性损伤炎症反应中起着重要作用。BACKGROUND：Cytokines secreted from neurons and glial cel s in early stage of spinal cord injury probably are essential factors for inducing secondary immunologic injury. OBJECTIVE：To investigate the effects of interleukin-1β, interleukin-6 and interleukin-17 on inflammatory reaction after acute spinal cord injury. METHODS：A total of 75 adult male Sprague-Dawley rats were randomly divided into control group and the spinal cord injury groups：1, 6, 24 and 72 hours. A rat model of incomplete spinal cord injury was established by the modified Al en weight drop method. The control group just underwent laminectomy. Injured spinal cord and spleen tissues were col ected at corresponding time points after model induction. Immunohistochemical staining was used to detect the distribution and expressions of interleukin-6 and interleukin-17 in spinal cord tissue. Western blot assay was utilized to detect the changes in p-STAT3 expression in injured spinal cord. RT-PCR was applied to measure the mRNA expression of interleukin-1β, interleukin-6 and interleukin-17 in the spleen. RESULTS AND CONCLUSION：The expression levels of p-STAT3, interleukin-1β, interleukin-6, and interleukin-17 were significantly higher in the spinal cord injury groups than those in the control group （P〈0.05）. The expression of inflammatory cytokines increased immediately after injury. Interleukin-1βand interleukin-6 levels peaked at 6 hours, and then decreased. p-STAT3 and interleukin-17 levels peaked at 24 hours, and then decreased. The expression was stil higher at 72 hours than that in the control group. Results suggested that the expression of p-STAT3-mediated pro-inflammatory cytokines interleukin-1βand interleukin-6 in early stage increased. Inflammatory cascade would enlarge in the injured area, which probably induced secondary spinal cord injury and increased interleukin-17 levels. These possibly played a key role in secondary inflammatory reaction.

关 键 词：实验动物 组织构建 白细胞介素1Β 白细胞介素6 白细胞介素17 P-STAT3 脊髓损伤 

分 类 号：R318[医药卫生—生物医学工程]