氢盐水可抑制脊髓缺血再灌注损伤兔模型运动神经元的凋亡  

Hydrogen-rich saline can inhibit apoptosis of spinal cord motor neurons in rabbits with spinal cord ischemia-reperfusion injury

作　　者：孙延卿[1] 陈雄生[1] 曹东[1] 朱巍[1] 贾连顺[1] 

机构地区：[1]解放军第二军医大学附属长征医院脊柱外科,上海市200003

出　　处：《中国组织工程研究》2014年第18期2861-2866,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:脊髓缺血再灌注损伤是一种严重的继发性脊髓损伤,其损伤机制是多因素综合作用的结果,其治疗上也有多种措施,但治疗效果不甚理想。目的:探讨氢盐水对脊髓缺血再灌注损伤兔模型运动神经元的保护作用及机制。方法:采用ZIVIN法制备脊髓缺血再灌注损伤兔模型,并采用氢盐水治疗(设为氢盐水组),同时设模型组和假手术组为对照。结果与结论:氢盐水组兔后肢运动功能Tarlov评分于再灌注后6,12,24,72 h明显优于模型组(P<0.01)。再灌注后72 h,与模型组相比,氢盐水组丙二醛浓度降低(P<0.05),过氧化氢酶活性升高(P<0.05)。苏木精-伊红染色显示,假手术组脊髓前角运动神经元细胞结构完整,模型组脊髓前角大量运动神经元细胞坏死,胞浆内颗粒变性和空泡变性。氢盐水组脊髓前角运动神经元细胞结构基本完整,仅有少量运动神经元细胞空泡变性。原位末端标记染色显示,假手术组未见运动神经元细胞凋亡;模型组见大量凋亡的运动神经元及大量炎性细胞浸润;氢盐水组见脊髓前角少量凋亡的运动神经元及少量炎性细胞浸润。结果证实,氢盐水可抑制兔缺血再灌注损伤脊髓运动神经元的凋亡,其机制与其抗氧化作用有关。BACKGROUND：Spinal cord ischemia-reperfusion injury is a serious secondary injury of the spinal cord. Multifactor could contribute to the mechanism of this injury, and many therapeutic measures emerge, but the therapeutic effect is not ideal. OBJECTIVE：To investigate the protective effects and mechanism of hydrogen-rich saline on spinal cord ischemia-reperfusion injury in rabbits. METHODS：ZIVIN method was adopted to prepare the model of spinal cord ischemia-reperfusion injury. The rabbit models were randomly divided into model group, sham operation group, and hydrogen-rich saline group. RESULTS AND CONCLUSION：Improved Tarlov scores for the evaluation of motor function were significantly increased in hydrogen-rich saline group compared with the model group at 6, 12, 24, 72 hours after reperfusion （P〈0.01）. The contents of malondialdehyde were significantly lower （P〈0.05）, while catalase activity was significantly higher （P〈0.05） in hydrogen-rich saline group than that in model group at 72 hours after reperfusion. Hematoxylin-eosin staining revealed that, spinal cord anterior-horn motor neurons maintained intact structure in sham operation group;more necrotic spinal cord anterior-horn motor neurons were found in model group, and granular-vacuolar degeneration occurred in the endochylema. In hydrogen-rich saline group, the structure of spinal cord anterior-horn motor neurons was basical y intact, only a smal amount of spinal cord anterior-horn motor neurons appeared vacuolar degeneration. TUNEL staining showed no apoptotic spinal cord anterior-horn motor neurons in sham operation group. Many inflammatory cel s and apoptotic neurons were found in model group. There were few inflammatory cel s and apoptotic neurons in hydrogen-rich saline group. Hydrogen-rich saline can prevent the apoptosis of spinal cord anterior-horn motor neurons in rabbits with spinal cord ischemia-reperfusion injury, and the underlying mechanism is associated with antioxidative effect.

关 键 词：实验动物 组织构建 组织构建基础实验 氢盐水 脊髓损伤 缺血再灌注 运动神经元 凋亡 抗氧化 腹腔注射 模型 运动功能 保护 

分 类 号：R318[医药卫生—生物医学工程]