体外受精-胚胎移植的助孕效果:胚胎体外培养和低温保存  

Reproductive effects of in vitro fertilization-embryo transfer: in vitro culture and cryopreservation of embryos

作　　者：王芳[1] 陈绍威[1] 

机构地区：[1]四川省泸州医学院附属医院生殖医学技术部,四川省泸州市646000

出　　处：《中国组织工程研究》2014年第18期2939-2944,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:从第一例试管婴儿诞生至今已二十余年,体外受精-胚胎移植技术在世界范围内得到蓬勃发展。目的:回顾性分析体外受精-胚胎移植的助孕效果,探讨体外受精胚胎的培养及低温保存技术的应用。方法:2013年泸州医学院附属医院生殖医学技术部接受体外受精-胚胎移植或卵细胞浆内单精子注射助孕的不孕患者131例,共147个取卵周期,124个移植周期,患者平均年龄31.32岁。有131个取卵周期应用标准长方案促排卵,4个取卵周期应用超长方案促排卵、10个取卵周期应用超短方案促排卵,2个取卵周期为自然周期排卵;应用新鲜胚胎移植93例,冷冻胚胎移植28例。观察不同促排卵方案、新鲜或冷冻胚胎移植患者的临床妊娠率、胚胎植入率和流产率等。结果与结论:应用标准长方案、超长方案及超短方案促排卵患者的临床妊娠率为34.7%,50%,20%;植入率分别为19.1%,22.2%,30%;流产率为21.7%,50%,33.3%;以上指标自然周期排卵患者均为0。新鲜胚胎移植临床妊娠率33.6%,植入率18.1%,流产率20.1%;冷冻胚胎移植临床妊娠率53.8%,植入率22.3%,流产率20.7%。提示应用标准长方案促排卵效果好,新鲜及冷冻胚胎移植的疗效相当,胚胎的体外培养及低温冷冻是辅助生殖技术中重要步骤,需进一步深入研究。BACKGROUND：Since the first successful birth of a＆quot;test tube baby＆quot;, in vitro fertilization-embryo transfer technology has been greatly developed within 20 years. OBJECTIVE：To retrospectively analyze the assisted reproductive effects of in vitro fertilization-embryo transfer, and to explore the culture of embryos in vitro and the application of cryopreservation technology. METHODS：A total of 131 infertile patients received in vitro fertilization-embryo transfer or intracytoplasmic sperm injection in Department of Reproductive Medicine, Affiliated Hospital of Luzhou Medical Col ege, China. There were 147 oocyte retrieval cycles and 124 transfer cycles, the mean age of the patients was 31.32 years. Standard long-protocol ovulation induction was given to 131 oocyte retrieval cycles, super-long-protocol ovulation induction was given to 4 oocyte retrieval cycles, and super-short-protocol ovulation induction was given to two oocyte retrieval cycles;93 cases were transplanted with fresh embryos and 28 cases with frozen embryos. The clinical pregnancy rate, embryo implantation rate and abortion rate of patients receiving different ovulation induction protocols and fresh or frozen embryo transfer were observed. RESULTS AND CONCLUSION：In the patients receiving standard long, super-long and super-short protocols for ovulation induction, the clinical pregnancy rate was respectively 34.7%, 50%, 20%;the embryo implantation rates were 19.1%, 22.2%, 30%;and abortion rate was 21.7%, 50%, 33.3%;in those patients, no case exhibited natural cycle of ovulation. In the patients receiving fresh embryo transfer, the clinical pregnancy rate was 33.6%, embryo implantation rate was 18.1%, and abortion rate was 20.1%;in frozen embryo transferred patients, the clinical pregnancy rate was 53.8%, embryo implantation rate was 22.3%, and abortion rate was 20.7%. Experimental findings indicate that, standard long-protocol ovulation induction has good effects, the efficacy of fresh embryo transfer is equivalent to that of fro

关 键 词：实验动物 组织构建 体外受精 胚胎移植 不孕 胚胎 体外培养 低温保存 促排卵 临床妊娠率 

分 类 号：R318[医药卫生—生物医学工程]