纳米Ag-SiO_2导尿管对人体外尿道黏膜细胞的毒性  

Cytotoxicity evaluation of nano-Ag-SiO_2 urethral catheter for external urethral mucosa cells

作　　者：张展[1] 刘春[2] 

机构地区：[1]山西医科大学,山西省太原市030001 [2]山西医科大学第一医院,山西省太原市030001

出　　处：《中国组织工程研究》2014年第16期2550-2556,共7页Chinese Journal of Tissue Engineering Research

基　　金：山西省自然科学基金资助项目(2006011119)~~

摘　　要：背景:纳米Ag-SiO2导尿管抗菌作用突出,明显减少了长期留置尿管所致的泌尿系感染。在追求纳米银医疗产品优异抗菌性的同时,人们也越来越重视其生物安全性。目的:将人前列腺部尿道上皮细胞置于纳米Ag-SiO2导尿管浸提液中培养,评价纳米Ag-SiO2导尿管的生物学安全性。方法:分别以普通导尿管浸提液、纳米Ag-SiO2导尿管浸提液及普通细胞培养液培养前列腺增生症患者前列腺部尿道上皮黏膜细胞,培养2,5,7 d,通过四甲基偶氮唑盐比色法(MTT),用酶标仪测定各培养间期各组体外细胞的吸光度值,计算细胞相对增殖率,同时通过倒置显微镜观察细胞的形态、生长和增殖情况,定量和定性地进行毒性评价及比较。结果与结论:纳米Ag-SiO2导尿管浸提液细胞毒性为1级,普通导尿管浸提液细胞毒性为0、1级,细胞相对增殖率组别效应无统计学差异(F=0.544,P=0.475);细胞相对增殖率时间效应有统计学差异(F=3.031,P=0.086);组间与时间之间交互作用无统计学差异(F=0.130,P=0.879)。结果表明纳米Ag-SiO2导尿管对不同培养间期细胞的生长繁殖影响微弱,符合医疗器械生物学评价标准。BACKGROUND：Nano-Ag is increasingly used as antibacterial materials in medical products due to its excelent antibacterial activity. Antibacterial activated nano-Ag-SiO2 OBJECTIVE： To evaluate the biosecurity of nano-Ag-SiO urethral catheter can reduce the incidence of catheter-associated urinarytract infections obviously. As more and more nano-Ag medical products have been developed, their biological security is becoming a focused issue. 2 urethral catheter through comparing the active influence of nano-Ag-SiO2 METHODS： Mechanical separation and enzymatic digestion were used to isolate and culture urothelial mucosa of benign prostatic hyperplasia patients to make cellsuspension after proliferationin vitro. The medium was replaced by nano-Ag-SiO urethral catheter and ordinary urethral catheter extracts onin vitro cels of human prostatic urethra epithelial cels in different periods. 2 and general catheter tube extracts, respectively. MTT assay was performed to quantify thein vitro cytotoxicity of two extracts on human urethra epithelial cels which were cultured for 2, 5 and 7 days. Absorbance values of nano-Ag-SiO2 RESULTS AND CONCLUSION：The cytotoxicity of extract of nano-Ag-SiO and general urinary catheter groups were measured by enzyme-linked immunosorbent assay to calculate the relative growth rate, and toxicity evaluation was compared and evaluated. Under an inverted microscope, cellmorphology, growth and proliferation were observed. 2 catheter was 1 grade, and that of general medical catheter extract was 0 to 1 grade. There was no statistical difference in group effect of relative proliferation rate （F=0.544,P=0.475）; there was statistical difference in time effect of relative proliferation rate （F=3.031,P=0.086）; no statistical difference was found in the interaction between group and time （F=0.130,＆amp;nbsp;P=0.879）. These findings indicate that nano-Ag-SiO2 catheter has no or low cytotoxicity effect on cellgrowth and proliferation, which is consistent with the biological evaluation sta

关 键 词：生物相容性材料 纳米粒 尿道上皮 二氧化硅 

分 类 号：R318[医药卫生—生物医学工程]