不同营养方式对小球藻FACHB 484生长的影响及其非自养生长机理研究  

STUDY ON THE EFFECTS OF DIFFERENT NUTRITION MODES ON THE GROWTH OF CHLORELLA SP.FACHB 484 AND THE MECHANISM OF HETEROTROPHIC GROWTH

作　　者：牛海亚 马玉龙[1,2] 石勋祥[1] 牛芮 

机构地区：[1]宁夏大学能源化工国家重点实验室培育基地,宁夏750021 [2]宁夏大学化学化工学院,宁夏750021

出　　处：《水生生物学报》2014年第3期474-479,共6页Acta Hydrobiologica Sinica

基　　金：国家973计划前期研究专项(No.2012CB723106);宁夏环境科学研究项目(No.20100803)资助

摘　　要：系统研究了小球藻FACHB 484在含有葡萄糖的不同营养方式下的生长情况,并通过抑制试验探讨葡萄糖在小球藻FACHB 484光异养和兼养生长条件下所起的作用以及小球藻FACHB 484是否存在氧化呼吸系统的关键酶类。结果表明:小球藻FACHB 484可利用葡萄糖进行化能异养、光激活异养、光异养及兼养生长,其生长速率大小为:兼养>光异养>光激活异养>化能异养>光合自养。兼养培养的最大生物量和比生长速率分别是自养培养的8.6和3.4倍,其比生长速率接近于光合自养和光异养培养下的比生长速率之和。葡萄糖主要作为小球藻FACHB 484兼养和光异养培养的碳源,而能量主要源自光。小球藻FACHB 484存在氧化呼吸链代谢途径,其细胞中有琥珀酸脱氢酶和细胞色素氧化酶。Here we systematically studied the growth of Chlorella sp.FACHB 484 in glucose-containing medium in different nutrition modes.We also conducted the inhibition tests to investigate the effects of glucose on the growth of Chlorella sp.FACHB 484 in heterotrophic and mixotrophic conditions,as well as to identify the key enzyme in oxidation respiratory system.We observed that Chlorella sp.FACHB 484 could utilize glucose as the source of nutrition in all the test nutritional modes and showed different growth rates in the order mixotrophy ＆gt; photoheterotrophy ＆gt; light-activated heterotrophy ＆gt; chemoheterotrophy ＆gt; photoautotrophy.The maximum biomass and specific growth rate（SGR） of the tested algae in mixotrophy were 8.6 and 3.4 times higher than those in photoautotrophy.The value of SGR of Chlorella sp.FACHB 484 in mixotrophy was close to the sum of the values of SGRs in photoautotrophy and photoheterotrophy.In mixotrophic and photoheterotrophic modes,Chlorella sp.FACHB 484 used glucose and light as the sources of carbon and energy respectively.The cells of Chlorella sp.FACHB 484 expresses succinic dehydrogenase and cytochrome oxidase which are most likely the key enzymes of the oxidation respiratory chain.

关 键 词：小球藻 异养生长 兼养生长 代谢机理 

分 类 号：S968[农业科学—水产养殖] Q949.2[农业科学—水产科学]