川中丘陵地区大豆根瘤菌遗传多样性与系统发育关系  

Genetic diversity and phylogeny of soybean rhizobia isolated from the Hilly Area of Central Sichuan in China

作　　者：熊峰[1] 陈远学[1] 张思兰[1] 游芳[1] 周欢[1] 徐开未[1] 

机构地区：[1]四川农业大学资源环境学院,四川成都611130

出　　处：《微生物学报》2014年第6期616-623,共8页Acta Microbiologica Sinica

基　　金：四川省科技支撑计划项目(2012RZ0018)~~

摘　　要：【目的】研究分离自川中丘陵地区大豆根瘤菌的遗传多样性和系统发育。【方法】采用16S rDNA PCR-RFLP和16S rRNA基因、glnII、共生基因(nodC)系统发育分析的方法进行研究。【结果】供试未知菌的16S rDNA用4种限制性内切酶(HaeⅢ、HinfⅠ、MspⅠ及TaqⅠ)酶切后获得5种16S遗传图谱类型。16S rDNA PCR-RFLP结果表明,所有供试菌株在83%水平分为慢生根瘤菌属(Bradyrhizobium)和中华根瘤菌属(Sinonrhizobium)两大类群,而75%的菌株为中华根瘤菌。6个代表菌株的16S rDNA、glnII和nodC三个位点基因的系统发育结果基本一致,4株与S.fredii USDA205T相似度最高;有2株分别与B.yuanmingense CCBAU10071T、B.diazoefficiens USDA110T相似度最高。4个Sinonrhizobium代表菌株16S rDNA、glnII序列相似度分别为98.3%-99.9%、98.2%-100%,但它们的nodC基因序列完全相同。【结论】川中丘陵地区大豆根瘤菌具有较丰富的遗传多样性,S.fredii为优势种。[ Objective] We investigated the genetic diversity and phylogeny of 28 rhizobial isolates from root nodules of soybean growing in the Hilly Area of Central Sichuan in China. [ Methods ] We used 16S rDNA PCR-RFLP and phylogenetic analyses of the 16S rDNA, glnll and symbiotic genes （nodC）. [ Results] Five 16S rDNA genotypes among the isolates were distinguished with restriction endonucleases HaeⅢ, Hinf Ⅰ , Msp Ⅰ and Taq Ⅰ . In the 16S rDNA PCR- RFLP analysis, all the isolates are divided into Bradyrhizobium group and Sinonrhizobium group at the 83% level, and Sinonrhizobium strains accounted for 75% of the isolates. The phylogenetic analyses of 16S rDNA , glnll and nodC show that 4 representative strains SCAUsl, SCAUs2, SCAUs7 and SCAUs4 were closely related to S. fredii USDA205Ywhile the other 2 representative strains SCAUs3 and SCAUs5 were closely related to B. yuanmingense CCBAU10071^T and B. diazoefficiens USDA110^T. The 16S rDNA, glnH and nodC sequence similarity of 4 Sinonrhizobium representative strains were 98.3 % -99.9 % , 98.2 % -100 % and 100%, respectively. [ Conclusion] Soybean rhizobia isolated from the Hilly Area of Central Sichuan in China has rich genetic diversity, S. fredii was the predominant genus.

关 键 词：大豆根瘤菌 PCR-RFLP 16S RDNA glnⅡ nodC 遗传多样性 

分 类 号：Q933[生物学—微生物学] S154.3[农业科学—土壤学]