细粒棘球蚴抗原B对1型糖尿病小鼠的保护作用  

Protective effect of Echinococcus granulosus antigen B on low-dose streptozotocin-induced diabetes mellitus in mice

作　　者：阿依甫汗.阿汗 哈丽娅[1] 吐尔干艾力.阿吉 李海涛[1] 邵英梅[1] 赵晋明[1] 温浩[1] 

机构地区：[1]新疆医科大学第一附属医院肝胆包虫外科,乌鲁木齐医学博士研究生830000

出　　处：《医学研究生学报》2014年第5期452-455,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金(30760239);新疆维吾尔自治区自然科学基金(2010211B21)

摘　　要：目的细粒棘球蚴感染存在自发性免疫调节现象,在人体内经历了从Th1向Th2的极化过程。文中探讨细粒棘球蚴抗原B对小鼠实验性自身免疫性1型糖尿病的保护作用及其机制。方法采用随机数字表法将30只雄BALB/c小鼠随机分为3组:肌内注射细粒棘球蚴抗原B+糖尿病成模组(实验组,n=10);肌注等渗盐水+糖尿病成模组(等渗盐水对照组,n=10);糖尿病成模组(阳性对照组,n=10);实验组、等渗盐水对照组、阳性对照组均给予小剂量链脲佐菌素(STZ)诱导1型糖尿病,动态观察血糖变化。至3周末留取小鼠血清后,取胰腺组织切片HE染色,进行胰岛功能评价,免疫组化染色,观察胰岛β细胞总量变化,用ELISA测定血清白介素-2(interleukin-2,IL-2)、IL-10水平,间接放射免法测定血清胰岛素。结果给药后,小鼠血糖逐渐升高,在第3天和成模后1周、2周、3周,等渗盐水对照组[(21.91±3.11)、(24.77±4.29)、(32.47±2.03)、(18.13±2.18)mmol/L]和阳性对照组[(22.63±3.55)、(22.89±5.69)、(29.37±3.37)、(29.57±5.37)mmol/L]血糖水平分别显著高于实验组[(16.19±4.58)、(18.82±2.15)、(21.66±1.63)、(20.76±2.29)mmol/L],差异有统计学意义(P<0.05);实验组胰岛可见淋巴细胞浸润不多,而等渗盐水对照组和阳性对照组胰岛可见较多淋巴细胞浸润;在模型建立3周后,实验组小鼠血清胰岛素水平[(35.10±3.86)mIU/L]高于等渗盐水对照组[(27.16±6.11)mIU/L]和阳性对照组[(25.13±4.16)mIU/L],差异有统计学意义(P<0.05),且实验组小鼠胰岛β细胞总量[(0.62±0.08)mg]高于等渗盐水对照组[(0.27±0.04)mg]和阳性对照组[(0.25±0.02)mg],差异有统计学意义(P<0.05);糖尿病模型建立3周末,实验组小鼠血清IL-10水平[(80.91±11.28)ng/mL]高于等渗盐水对照组[(32.70±6.57)ng/mL]和阳性对照组[(34.32±4.01)ng/mL],差异有统计学意义(P<0.05),实验组小鼠血清IL-2水平[(196.16±11.71)ng/mL]低于等渗盐水对照组[(267.30±69.28)ng/mL]和�Objective Echinococcus granulosus infection may regulate the spontaneous immune response of the body,in which the body experiences the polarization of the cytokine profile from Th1 into Th2.This study was to investigate the protective effect of Echinococcus granulosus antigen B against type-1 diabetes mellitus in rats and its possible mechanism.Met hods Using the random numerical table method,30 male BALB/c mice were equally divided into groups A （experimental）,B （normal saline control）,and C （positive control）.All the mice were intraperitoneally injected with low-dose streptozotocin to induce type-1 diabetes mellitus.Then the animals in group A were muscularly injected with Echinococcus granulosus antigen B,those in group B with normal sodium,and those in group C left untreated.After modeling,the dynamic changes in the blood glucose level were observed,and at 3 weeks,the mice were executed,their pancreases harvested and serum samples collected for evaluation of insulitis by HE staining,observation of changes in the islet β-cell mass by immunohistochemistry,determination of the concentration of serum insulin,and measurement of the levels of serum IL-10 and IL-4 by ELISA in different groups.Results After streptozotocin injection,the mice became gradually hyperglycemic.At 3 d,1 wk,2 wk,and 3 wk after modeling,the mean blood glucose levels were （16.19 ±4.58）,（18.82 ± 2.15）,（21.66 ± 1.63）,and （20.76 ±2.29） mmol/L in group A,significantly lower than in B （[21.91 ±3.11],[24.77 ±4.29],[32.47±2.03],and [32.42± 1.95]mmol/L） and C （[22.63 ±3.55],[22.89 ±5.69],[29.37 ± 3.37],and [29.57 ±5.37]mmol/L） （P〈0.05）.There were only a few islets with lymphocytic infiltration in group A,but a lot of islets with massive lymphocytic infiltration in B and C.At 3 wk,the serum insulin concentration and islet β-cell mass were significantly increased in group A （[35.10 ± 3.86] mIU/L and [0.62 ± 0.08] mg） as compared with B （[27.16 ±6.11] mIU/L and [0.27 ±0.04] mg） and C （

关 键 词：细粒棘球蚴抗原B 1型糖尿病 TH1 TH2 

分 类 号：R587.1[医药卫生—内分泌]