iTRAQ技术在急性脊髓损伤脑脊液蛋白质组学研究中的应用  

Application of iTRAQ in the study of cerebrospinal fluid proteomics after acute spinal cord injury

作　　者：周义军[1] 徐磊磊[1] 宋兴华[1] 丁俐文[1] 陈江涛[1] 王翀[1] 甘雨灵[1] 朱晓萌[1] 

机构地区：[1]新疆医科大学第一附属医院骨肿瘤科,乌鲁木齐医学硕士830000

出　　处：《医学研究生学报》2014年第5期464-468,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81160218)

摘　　要：目的急性脊髓损伤(acute spinal cord injury,ASCI)是一种严重的神经系统创伤。研究旨在建立ASCI后脑脊液(cerebrospinal fluid,CSF)差异蛋白质谱,从分子水平研究ASCI后继发性损伤的机制。方法运用SD大鼠建立ASCI模型,损伤后取CSF用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术鉴定大鼠ASCI后CSF的差异蛋白质。结果共鉴定到722个蛋白,其中差异表达的蛋白107个,包括63个下调的差异蛋白和44个上调的差异蛋白。其中相关炎症反应的差异蛋白13个、相关急性炎症反应的差异蛋白5个、相关慢性炎症反应的差异蛋白2个、调节炎症反应的差异蛋白7个、积极调节炎症反应的差异蛋白2个、消极调节炎症反应的差异蛋白2个。结论鉴定了ASCI后脑脊液差异蛋白及相关炎症因子,为ASCI后继发性损伤的机制和过程的动态监测及相关治疗靶点提供理论依据。Objective Acute spinal cord injury （ASCI） is a serious nervous system trauma.This study aimed to establish the protein spectrums of cerebrospinal fluid （CSF） differential proteins after ASCI and investigate the mechanisms of secondary ASCI at the molecular level.Methods We established ASCI models in SD rats,and then identified the differential proteins of CSF after ASCI using isobaric tags for relative and absolute quantitation （iTRAQ）.Results Totally,722 proteins were identified,including 107 differentially expressed proteins （sixty-three down-regulated and forty-four up-regulated）.Among them,there were thirteen related to inflammatory response,five to acute inflammatory response,two to chronic inflammatory response,seven to the regulation of inflammatory response,two to the positive regulation of inflammatory response,and two to the negative regulation of inflammatory response.Conclusion Differentially expressed proteins of CSF and their related inflammation factors after ASCI were identified,which has provided some theoretical evidence for the study of the mechanisms,dynamic monitoring of the process,and related therapeutic targets of secondary spinal cord injury.

关 键 词：急性脊髓损伤 脑脊液 蛋白质组学 ITRAQ 

分 类 号：R651.2[医药卫生—外科学]