原花青素对H_2O_2引起PC12细胞的内质网应激损伤的保护作用  

Protective effects of proanthocyanidins on the endoplasmic reticulum stress in PC12 cells induced by H_2O_2

作　　者：马玉红[1] 张梦莹[2] 所起凤[2] 钟民[2] 吕坤[2] 

机构地区：[1]皖南医学院诊断学教研室 [2]皖南医学院弋矶山医院中心实验室

出　　处：《中国临床药理学与治疗学》2014年第4期389-392,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省高校高等学校省级优秀青年人才基金项目(2012SQRL124);皖南医学院中青年基金资助(WK201019)

摘　　要：目的:研究H2O2诱导PC12细胞的内质网应激损伤作用及原花青素对其预防作用。方法:H2O2100μmol/L诱导PC12细胞损伤18h复制阿尔茨海默病模型(AD),原花青素40mg/L、SB203580 10μmol/L、Tempol800μmol/L于造模前30min预处理后,RTPCR检测BiP/GRP-78mRNA表达水平变化;流式细胞术检测细胞内反应氧产物(ROS)阳性细胞生成率,计算平均荧光强度。结果:H2O2100μmol/L诱导PC12细胞损伤后BiP/GRP-78mRNA表达量显著增高,ROS生成增加;原花青素40mg/L可显著降低BiP/GRP-78mRNA表达水平(P<0.01),减少细胞内ROS生成(P<0.01)。结论:原花青素可减轻H2O2引起PC12细胞的内质网应激损伤。AIM： To study the protective effects of the proanthocyanidins on the endoplasmic reticulum stress in PC12 cells induced by H202. METHODS： The models of alzheimer＇s disease were established by treating PC12 cells with H202（100 μmol/L）for 18 h. Before modeling for 30 minutes proanthocyanidins 40 mg/L, SB203580 10 μmol/L, Tempol 800 μmol/L were administrated respectively. The level of BiP/ GRP-78 mRNA was detected by RT-PCR. The formation rate of reactive oxygen species（ROS） positive cells was detected with flow cytometry, the mean fluorecence was calculated. RESULTS：The level of BiP/GRP-78 mRNA and the formation rate of ROS were decreased in PC12 cells treated with H2O2; the level of BiP/GRP-78 mRNA and the formation rate of ROS were decreased（P〈0.01） with proanthocyanidins treatment （40 mg/L）in PC12 cells treated with H202. CONCLUSION Proanthocyanidins can ameliorate endoplasmic reticulum stress in PC12 induced bywith H2O2.

关 键 词：原花青素 过氧化氢 PC12细胞 BIP GRP-78 反应氧产物 

分 类 号：R965.2[医药卫生—药理学]