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作 者:刘亚莉[1] 李运生[1,2,3] 章美林[1,4] 陈真[1] 詹年[1] 章孝荣[1,2,3] 刘亚[1,2,3] 张运海[1] 杨宏星[1] 吴蓉花[1] 方富贵[1,2,3] 曹鸿国
机构地区:[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽地方畜禽遗传资源保护与生物育种省级实验室,安徽合肥230036 [3]安徽省羊繁育工程技术研究中心,安徽合肥230036 [4]安徽省妇幼保健医院,安徽合肥230000
出 处:《黑龙江农业科学》2014年第6期77-81,共5页Heilongjiang Agricultural Sciences
基 金:安徽省自主创新专项资助项目(12z010244)
摘 要:为获得更多安徽白山羊受精卵,以开展转基因育种工作,研究了不同孕酮阴道栓来源、季节以及供体妊娠状态对安徽白山羊超数排卵效果的影响。结果表明:使用国产孕酮阴道栓(CIDR)对供体母羊进行同步发情处理,头均排卵数和回收受精卵数分别为15.0±7.62和14.6±7.88枚,受精率为97.3%,而应用进口孕酮阴道栓对供体山羊进行同步发情处理,头均排卵数和回收受精卵数分别为11.0±5.38和10.8±5.16枚,受精率为98.2%,两组间差异不显著(P>0.05);安徽白山羊在春、秋、冬3个季节超数排卵,头均回收卵子数分别为13.71±6.28、16.54±7.43和13.95±6.15枚,季节间差异不显著(P>0.05);妊娠母羊和未妊母羊头均排卵数分别为14和15.2枚,两者差异不显著(P>0.05),但妊娠组回收卵子的受精率显著低于空怀组(P<0.05)。To obtain more oocytes from Anhui white goat for transgenic study, the effects of season, dornors' pregnency and the source of CIDR on superovulation in Anhui white goat were investigated. The results showed that when synchronized with domestic CIDR,average number of ovulated oocytes and colleted fertilized oocytes per donor were 15.0±7.62 and 14.6 ± 7.88 respectively, and fertilization rate was 97.3 % ,while synchronized with imported CIDR, average number of ovulated oocytes and eolleted fertilized oocytes per donor were 11.0 ± 5.38 and 10.8±5.16 respe.ctively, and fertilization rate was 98.2%, there was no significant difference between the two groups(P〉0.05) ; Average number of collected oocytes per donor were 13.71 ± 6.28,16.54±7.43 and 13.95±6.15 respectively when superoulated in spring, autumn and winter, there was no significant difference between any seasons (P 〉 0. 05) ; Average number of ovulated oocytes in pregnant and nonpregnant dornors were 14.0 and 15.2 respectively,there was no significant difference between two groups,but the fertilization rate of oocytes collected from pregnant dorors was lower than that of non-prenant dornors significantly(P〈0.05).
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