红花注射液对人肝癌HepG-2细胞AKT表达的影响  

Effects of Safflower injection on the expressions of AKT in human hepatoma HepG-2 cells

作　　者：李福娟[1,2] 唐小云[3] 桂金秋[3] 王小花[1] 李玉婷[1] 张涛[4] 石学魁[3] 

机构地区：[1]牡丹江医学院病原生物学与免疫学实验室,黑龙江牡丹江157011 [2]佳木斯大学 [3]牡丹江医学院病原生物学教研室,黑龙江省高校肿瘤疾病防治重点实验室,黑龙江牡丹江157011 [4]佳木斯大学基础医学院免疫教研室,黑龙江佳木斯154007

出　　处：《广东医学》2014年第10期1487-1490,共4页Guangdong Medical Journal

基　　金：黑龙江省教育厅科研项目(编号:11551520)

摘　　要：目的探讨红花注射液对肝癌HepG-2细胞增殖的影响及分子机制。方法应用红花注射液(0、50、150、250 g/L)处理HepG-2细胞,经AnnexinⅤ-FITC/PI双染荧光显微镜观察细胞凋亡的形态学改变,实时荧光定量PCR检测AKT mRNA表达,蛋白质印迹(Western blot)检测AKT、p-AKT蛋白表达。结果 50、150、250g/L的红花注射液作用于HepG-2细胞48 h后,荧光显微镜下呈现典型的凋亡细胞形态;细胞内AKT mRNA表达水平显著降低,AKT、p-AKT蛋白表达水平也显著下调,且变化趋势均呈一定的剂量依赖关系。结论红花注射液可能通过抑制AKT信号通路,阻碍肝癌HepG-2细胞的增殖及诱导凋亡。Objective To investigate the effect and mechanism of Safflower injection on cell proliferation of human hepatoma cells HepG-2 in vitro.Methods HepG-2 cells were treated by different concentrations （0, 50, 150, 250 g/L） of safflower injection .Fluorescence microscopy was applied to test the morphological changes of apoptosis by AnnexinⅤ-FITC/PI staining.Quantitative real-time PCR was carried to assess the mRNA expression of AKT.Western blot were used to observe the protein expression levels of AKT and p -AKT in HepG-2 cells.Results After treated with Safflower injection , HepG-2 cells presented the typical modality of early apoptosis; with significantly dose -dependent down-regulation in AKT mRNA, and AKT and p-AKT proteins.Conclusion Safflower injection inhibits cell prolifera-tion and induces apoptosis in HepG -2 cells through AKT signaling pathway .

关 键 词：红花注射液 人肝癌细胞株HepG-2 细胞增殖 细胞凋亡 AKT 

分 类 号：R382.5[医药卫生—医学寄生虫学]